Now there are four main kinds of RNA interference libraries with different construction approaches and molecular form,chemically synthesized small interference RNA(siRNA) libraries,chemically synthesized small hairpin RNA(shRNA) libraries,enzymatically prepared siRNA libraries and enzymatically prepared shRNA libraries.
不同种类的RNA干扰库已经被用于基因功能的研究之中,根据其构建方式和分子形式的不同,可将RNA干扰库分为4种类型,即化学合成的小干扰RNA(siRNA)库、化学合成的小发夹RNA(shRNA)表达库、酶切法构建的siRNA库和酶切法构建的shRNA表达库。
[Methods] A shRNA expressed vector that expresses the specific small hairpin RNA targeting TRAP mRNA was constructed and transfected into SGC-7901 cells, and the stably expressing shRNA cells were selected by G418 and continuously cultured in half the antibiotic concentration for 3 months.
目的探讨稳定转染靶向端粒酶调节相关基因TRAP的小发夹RNA对胃癌细胞株SGC-7901的长效影响。
[Methods] Plasmid vectors expressing small hairpin RNA (shRNA) targeting at cyclin D1 gene were constructed and transfected into K562 cells mediated by chitosan.
[方法]体外构建靶向cyclin D1基因的小发夹RNA(shRNA)表达质粒,通过壳聚糖介导转染K562细胞,Western blot分析检测转染前后cyclin D1蛋白表达变化;集落形成实验检测细胞增殖能力;流式细胞仪检测细胞周期分布及凋亡情况。
Construction Expressing Vector of shRNA Inhibiting Cyclin D1 Gene Expression by RNA Interfering;
抑制cyclin D1基因小发夹RNA(shRNA)表达质粒的构建
[Methods] The 60nt nucleiotide sequence encoded targeting EBNA2 shRNA was cloned into a retroviral vector pSUPER.
[方法]用DNA重组技术将60nt能转录产生靶向EBNA2小发夹RNA(smallhairpin RNA,shRNA)的寡核苷酸序列定向插入逆转录病毒载体pSUPER。
Objective:To construct and identify the small hairpin RNA(shRNA) expression vector for Smad 4/DPC 4 gene.
目的:构建Smad4基因小发夹RNA(shRNA)表达载体。
Small splint immobilization for treatment of earthquake casualties
地震伤员小夹板固定治疗
For a long time,there is a firm problem about the application of a small splint that adjustment of cincture’s tightness degree is decided by the doctor s feeling and experience.
医用小夹板是四肢骨折康复治疗的重要器械。
a small clasp used by women and girls for holding the hair in place.
一种小夹子,被妇女和女孩用来夹住头发。
small splintage of humeral shaft fracture
肱骨干骨折小夹板固定术
small splintage of femoral shaft fracture
股骨干骨折小夹板固定术
The thief frisked her of her wallet.
小偷窃走了她的皮夹。
a thief lifted my wallet.
小偷扒走了我的钱夹
Do you have this jacket in a small?
这种夹克小号的有没有？
small ring-shaped stuffed pasta.
小的、环形的夹馅的面食。
cookies containing chocolate chips.
夹巧克力片的小甜饼。
A pocket-sized folder or case used to hold money and papers; a billfold.
皮夹子，钱包用来装钱和纸张的口袋大小的夹子或盒子；钱夹
The work-piece must be clamped tightly in jaw vice. Be careful and do not hurt fingers when fixing small work-pieces.
工件必须牢固地装夹在虎钳上，装夹小工件时须防止钳口夹伤手指。
A small writing board with a spring clip at the top for holding papers or a writing pad.
写字夹板一种小的写字板，顶部有一个弹簧夹，可以夹纸或书写笺
a small writing board with a clip at the top for holding papers.
一个很小的书写板，顶部有一个夹子，可用来夹纸。
The Research on Damage Monitoring in Smart FRP Sandwich Beam and Plate by Wavelet Analysis;
FRP夹层梁及夹层板损伤检测的小波分析研究
Parentheses: Round brackets.
圆括号（小括号）（夹注号）:圆的括号。亦称小括号、夹括号。
In the rat-trap, sir," replied the boy.
在捕鼠夹上，先生。”那小男孩说。
lapel mike
可夹在口袋或衣襟上的小型麦克风
Jenny scorch her jacket carelessly while she is smoking her pipe .
珍妮抽烟斗时不小心把夹克烧焦。
a wallet, chequebook, diary, fountain-pen, knife,
一个钱夹、支票簿、日记、自来水笔、小刀、