Effects of four detergents on solubilization and denaturation of microsomal cytochrome P450s in Helicoverpa armigera;
四种去垢剂对棉铃虫微粒体细胞色素P450的增溶与变性作用
Methods Human skeletal muscle proteins were extracted by using two zwitterionic detergents(3-[(3-cholamidopropyl) dimethylamino]-1-propane sulfonate,CHAPS;N-decyl-N-N′-dimethyl-3-ammonio-1-propane sulfonate,SB3-10) in different concentration of chaotrops.
方法使用两性离子去垢剂CHAPS和SB3-10,并配合不同浓度的离液剂抽提骨骼肌蛋白质,并通过双向电泳对蛋白质进行分离,用基质辅助激光解析电离飞行时间质谱(MALDI-TOF)对选取的蛋白质进行鉴定。
In this paper the effect of gangliosides on the erythrocte membrane injured by detergent Triton X- 100 is studied.
观察了神经节苷脂对去垢剂Ｔｒｉｔｏｎ—Ｘ—１００损伤大鼠红细胞膜的影响。