Methods Forty-eight samples from patients with ovarian tumor at different clinical stages and 8 from normal ovaries were examined for NM23-H1B mRNA expression by using RT-PCR, northern blot and in situ hybridization.
采用RT-PCR技术、RNA印迹(northern blot)法、原位杂交实验,检测NM23-H1B基因mRNA的表达。
METHODS: The validity and reliability of the results gotten by serial analysis of gene expression method was verified by RT-PCR and Northern blot.
以管家基因磷酸甘油醛脱氢酶(GAPDH)和肌动蛋白(β-actin)的mRNA表达水平为对照,比较了在JT库中表达水平比在WY库中高8倍的JT8标签对应基因的表达。
Methods: Total RNA was isolated from peripheral leukocytes of healthy men and women, AR mRNA was determined by RT PCR and Northern blot.
方法 :采用 RT- PCR和 Northern印迹方法检测健康成年人外周血白细胞 AR m RNA的表达。
Extraction and Yield of Total RNA in Cochleas of Normal Rats and Long-Term Sodium Salicylate Treated Rats;
正常及慢性水杨酸钠作用后大鼠耳蜗总RNA的提取和产量
By using the modified CTAB method, total RNA was isolated from Camellia oleifera leaves rich in such secondary products as polyphones and polymeric carbohydrates, and then mRNA was isolated and purified through double selections using oligo(dT) cellulose.
利用改良的CTAB法,从富含酚类糖类等次生物质油茶叶片中分离出总RNA,并从总RNA中,通过两次过Oligo(dT)柱分离纯化得到mRNA。
alternative RNA splicing
可变RNA剪接，旁路RNA剪接
premessenger RNA
前信使RNA,前mrna
Circulating RNA and miRNA in Blood:Potential Applications as Tumor Markers
外周血循环RNA、微小RNA与肿瘤诊断
Initiation of Genomic RNA Replication by a Recombinant Classical Swine Fever Virus RNA-dependent RNA Polymerase;
猪瘟病毒RNA依赖的RNA聚合酶起始基因组RNA复制的分子机制研究
Northern blotting
Northern印迹（法），RNA印迹（法）
Messenger RNA is templated along only one of the two strands of a DNA molecule.
DNA分子两条作为RNA模板。
In recent years, the phenomenon of RNA interference (RNAi) in cells was found.
近几年，细胞内RNA干扰(RNA interference，RNAi)现象被发现。
A third class of RNA is also required for protein synthesis, the ribosomal RNA.
蛋白质合成还需要第三类RNA，即核糖体RNA。
Activity Assay and Inhibitor Screening of HCV RDRP;
HCV RNA依赖的RNA聚合酶（RDRP）活性测定及抑制剂筛选
From Antisense RNA to RNA Interference: an revolutionary breakthrough led by opportunity and innovation;
从反义RNA到RNA干扰:科学突破的机遇与创新
Investigate the Biogenesis of CML with Small RNA and RNAi;
应用小RNA及RNA干扰研究慢性髓性白血病的致病机理
Construction of a Series of RNA Interference Vectors and Their Application in the Study of Genes Involved in the Invasion and Metastasis of Hepatocellular Carcinoma;
RNA干涉技术平台的建立及肝癌侵袭转移相关基因RNA干涉研究
Isolation and Function Identification of a Novel RNA-dependent RNA Polymerase Gene, GhRdRP, in Cotton (Gossypium Hirsutum L.);
棉花RNA依赖的RNA聚合酶（GhRdRP）基因的分离及功能分析
Cloning and Expression Analysis of an RNA-dependent RNA Polymerase Gene, NgRDR1, from Nicotiana Glutinosa
心叶烟RNA依赖的RNA聚合酶基因（NgRDR1）的克隆及其表达特性研究
The Expression and Purification of SARS-CoV RNA Dependent RNA Polymerase
SARS冠状病毒RNA依赖的RNA聚合酶原核和真核表达及纯化
Three Commercial RNA Extraction Methods in Hepatitis C Viral Load Test:Their Comparative Evaluation
3种RNA提取方法在血清丙型肝炎病毒RNA定量检测中的性能评价
Northwestern blotting
Northwestern印迹（法），RNA-蛋白质印迹（法）
external guide sequence
外部指导序列[如见于RNA酶P的反应]