Construction of SFV RNA replicon-based nucleic acid vaccine pIRSFV-HN and its anti-tumor effect in vivo and in vitro;
基于SFV RNA复制子核酸疫苗pIRSFV-HN的构建及其体内外抑瘤作用
RNA replicon-based vaccine, which is constructed by the viral RNA that can replicate by itself, is a newly springing up vaccine.
最近兴起的RNA复制子疫苗,利用源自病毒的能够自主复制的RNA,其结构蛋白基因由外源抗原基因取代,保留了非结构蛋白(RNA复制酶)基因。
The elements for Semliki forest virus (SFV) RNA replicon, a kind of new generation vector, comes from the Alphavirus genus.
新一代Semliki森林病毒RNA复制子源自甲病毒属(Alphavirus)Semliki森林病毒的基因组,它克服了一些现有的质粒载体表达效率低的缺点。
Cloning of Qβ RNA replicase cDNA gene and its expression in E.coli;
Qβ噬菌体RNA复制酶基因的克隆及在大肠杆菌中的表达
Objective To explore the enhancing effect of RNA replicase of alpha virus on the immune effect of genetic vaccine in order to obtain its optimal form.
目的 探讨α 病毒RNA复制酶对基因疫苗的免疫学效应的加强作用 ,寻找更好的基因疫苗形式。
coli expression system and in vitro RNA replication sys.
在合适的反应条件下NS5B可以多种RNA分子为模板催化RNA复制 ,特别是能有效复制HCV全长 (+ )RNA 。
The RNA replicase gene of foot-and-mouth virus was amplified and cloned into prokaryotic expression vector pET-32a(+).
利用 RT- PCR扩增了口蹄疫病毒 (FMDV )的 RNA复制酶基因 ,并将其克隆到原核表达载体 p ET- 32 a(+)中。