Secreted Expression of Mature Peptide Gene of Human Bone Morphogenic Protein-7 in Pichia pastoris;
人骨形成蛋白-7成熟肽基因在巴斯德毕氏酵母中的分泌表达
Cloning and Expressing of gsh 1 gene in Pichia pastoris;
gsh 1基因的克隆及其在巴斯德毕赤氏酵母中的表达
Expression of Monellin, a Sweet Protein, in Pichia pastoris;
甜蛋白Monellin在巴斯德毕赤氏酵母中的胞内表达
Objective:Construction of one novel recombinant Pichia pastoris for production of glutathione,and to study its glutathione synthesis capacity.
目的:构建一株新型的产谷胱甘肽的重组巴斯德毕赤氏酵母,并研究该重组菌合成谷胱甘肽的能力;方法:利用PCR技术克隆来源于酿酒酵母的γ-L-谷氨酰-L-半胱氨酸合成酶编码基因GSH1和谷胱甘肽合成酶的基因GSH2,串联插入表达载体pGAPZA,转化Pichia pastoris GS115,在Zeo-cin平板上挑选阳性转化子,并对阳性转化重组菌进行摇瓶培养筛选。
Oxygen uptake and transfer in recombinant Pichia pastoris cultivation;
氧在重组毕氏酵母发酵过程中的摄取与传递
Specific Growth Rate Control for Pichia pastoris Fed-Batch Cultivation;
毕氏酵母流加发酵过程的比生长速率控制
Expression of NS5ATP13 transregulated by HCV NS5A in Pichia pastoris;
HCV NS5A反式激活基因NS5ATP13在毕氏酵母中的表达研究
Clinical study on stomal ulcer of gastroduodenal benign diseases after operation;
4% ,毕氏Ⅰ式占 3 2 。
Metabolic Model and Oxygen Transfer Model in Recombinant Pichia Pastoris Cultivation;
重组毕氏酵母发酵过程的代谢—氧传递组合模型
Construction and expression of recombinant Pichia pastoris for pPIC9K/angiopoietin-1
pPIC9K/Ang-1毕氏酵母表达载体的构建及体外表达
Based on the morphological characteristics of budding yeast, a cell cycle model during the P. pastoris fed-batch cultivation was proposed.
基于芽殖酵母细胞形态的特征，提出了毕氏酵母甘油流加发酵过程的细胞循环模型。
Expression of Human SolubleCD40 Ligand in Pichia pastoris and Study on Its Biological Functions;
人可溶性CD40配体在毕氏酵母中的高效表达及其生物学功能的研究
Expression of Escherichia Coli Choline Dehydrogenase in Pichia Pastoris;
大肠杆菌胆碱脱氢酶基因在毕氏酵母（pichia pastoris）中的表达
Expression,Purification and Crystallization of the Catalytic Domain of Inactivating Urokinase-type Plasminogen Activator in Pichia pastoris
尿激酶催化结构域S356A突变体在毕氏酵母中的表达、纯化及结晶
Studies on Cloning, Expression and Immunological Reaction of the Gene Encoding Thioredoxin of Chinese Stain of Schistosoma Japonicum in Pichia Pastori;
日本血吸虫大陆株硫氧还蛋白在毕氏酵母菌的克隆表达及其免疫反应性研究
Expression of recombination human IL-13 in Pichia pastoris and its effect on the induction of DC from;
人重组白介素13在毕氏酵母中的表达及其体外诱导DC分化作用的研究
Expression of Glycolate Oxidase Gene in Pichia Pastoris;
乙醇酸氧化酶基因在毕赤氏酵母中的表达
Expression of Turbot Hepcidin Gene in Pichia Pastoris;
大菱鲆hepcidin基因在巴氏毕赤酵母中的表达研究
Secretory Expression of Recombinant Human Insulin in the Pichia Pastoris
重组人胰岛素在毕赤氏酵母中的分泌表达
Expression of Gene Encoding Alkaline Cellulose in Pichia pastoris and Fermentation of Recombinant Pichia pastoris
碱性纤维素酶基因在巴氏毕赤酵母中的表达及重组酵母菌发酵工艺的优化
Expression of Extracellular Domain of Human Vascular Endothelial Growth Factor Receptor-2 in Pichia Pastoris;
人血管内皮生长因子受体2胞外段在毕赤氏巴斯德酵母中的表达
Fermentation Conditions Study of Producting Recombination Human-like Collagen in Pichia Pastoris;
重组人胶原蛋白在巴氏毕赤酵母中分泌表达条件研究
Construction and Expression of the Vector with Multi-Copy hEGF Gene in Pichia Pastoris;
人表皮生长因子（hEGF）串联体的构建及在毕赤氏酵母中的表达
Study on Cloning and Expression of Trichoderma Reesei Cellulase Genes in Pichia Pastoris;
里氏木霉纤维素酶基因的克隆及其在毕赤酵母中表达的研究
Expression of Mannanase Gene man1 from Trichoderma Reesei RUT-C30 in Pichia Pastoris;
里氏木霉RUT-C30甘露聚糖酶基因man1在毕赤酵母中的高效表达
The Expression of EGⅢ in Pichia Pastoris and the Kinetics of Cellulose Hydrolyze by EGⅢ;
内切纤维素酶EGⅢ在毕赤氏酵母中的表达及其吸附催化动力学研究