Objective To construct anti-tumor recombinant toxin with PE40 as toxic part and study its cytotoxic effect.
方法利用基因工程技术将α促黑激素(-αmelanoma stimulating hormone,-αMSH)与绿脓杆菌外毒素A的部分重组基因,克隆到高效表达载体pET-20b中,表达出重组蛋白MSH-PE40,进行头颈部肿瘤细胞的体外杀伤实验。
Objective: In order to research on the highest expression of recombinant diphtheria toxin DAB389-(Gly4Ser)2-α-melanophore-stimulating hormone,the optimezed fermentation conditions of recombinant bacteria were studied.
目的:为了提高融合蛋白白喉毒素DAB389-(Gly4Ser)2-α-促黑激素的表达量,研究工程菌发酵条件的最优化。