To clone and characterize the avirulence gene cluster of Avr-Pi1, Avr-Pi2 and Avr-Pi4a, genomic DNAs of Magnaporthe grisea strains FJ81278ZB15, GUY11 and their F1 progenies were isolated and used as templates to screen for tightly linked random amplified polymorphic DNA(RAPD) markers .
为克隆稻瘟菌无毒基因簇Avr-Pi1、Avr-Pi2和Avr-Pi4a,以RAPD方法对稻瘟菌(Magnaporthegrisea)菌株FJ81278ZB15、GUY11及其F1后代群体进行扩增,得到2个与该无毒基因簇连锁的分子标记P1414700和P1389420。