Chicken insulin like growth factor Ⅰ(IGF?Ⅰ)labeled by 125 I was incubated with IGF?Ⅰ degradation enzyme(crude extraction from kidney)under various conditions,such as incubation time,temperature,pH,and content of crude enzyme.
结果显示 :①IGF Ⅰ分解酶反应时间以 30min为宜 ;②粗酶液的含量宜在 1 0 8μg·μL-1以下 ;③酶活性在 4 5℃时最高 ;④此酶在 5 0℃以下稳定 ,当温度升至 5 5℃时其活性急剧下降 ,升至 6 0℃时将降至最大活性的 2 0 %左右 ;⑤此酶最适pH值为 6 5～ 7 0。
This article deals with the bletting phenomeonon of the peach at different tempera- tures from the pectin type and the change in pectinestrase.
本文对桃子在不同温度的软化现象从果胶组成及果胶分解酶活性的变化进行研究,找出了在高温时抑制软化的最佳温度。
Objective In order to study the construction of the glucoside chain of Spirometra erinaseienroaei plerocercoid protease.
目的 研究曼氏裂头蚴蛋白分解酶结合糖链的构成。