Cloning and Functional Analysis of Maize Starch Branching Enzyme Gene SBEⅡb Promoter;
玉米淀粉分支酶SBEⅡb基因启动子的克隆与功能分析
Three winter wheat cultivars containing high, middle and low gluten planting in the clay, loam and sandy soil were used to study the changes of starch branching enzyme(SBE) activity in kernel during grain filling stage.
种植在粘土、壤土和沙土中的 3个面筋含量高、中、低的冬小麦品种灌浆期间籽粒中淀粉分支酶 (SBE)活性变化均呈单峰曲线 ,开花后 2 0d达到峰值。
The starch branching enzyme bound to starch granule in rice endosperm was investigated in the present paper.
对水稻胚乳淀粉颗粒结合的淀粉分支酶进行了研究。
As the targeted sequences of RNAi,parts of sbeⅡb genes encoding starch branching enzyme in maize was ascertained by searching internet database http:jura.
edu/bioc/siRNAext/,确定玉米淀粉分支酶SBEⅡb的部分基因序列作为RNAi的目标序列,通过RT-PCR方法从玉米自交系‘综31’中分别成功克隆出了玉米淀粉分支酶sbeⅡb基因的部分序列及其启动子序列,从大肠杆菌中成功克隆了木糖异构酶基因xylA作为筛选标记,构建了含有35S驱动的木糖异构酶基因和sbeⅡb启动子驱动的含有sbeⅡb目标序列的反向重复序列的表达载体pBAC413,并对玉米自交系实施转化。
By using Pollen Tube Pathway Method,the antisense gene of maize-starch branching enzyme(sbe2b)was transferred into maize inbred lines in order to depress Amylopectin formation and increase amylose content.
以利用花粉管通道法转化玉米淀粉分支酶基因的反义表达载体的后代中的PCR阳性植株为试材,分析其直链淀粉含量的变异。
p13W2, p13W4 and p13B4, containing the sense waxy gene, antisense waxy gene and antisense sbel gene respectively, were constructed and introduced into various rice cultivars, both of Japonica and Indica, via Agrobacterium.
2、为改变稻米胚乳中的直链淀粉含量，采用不同策略，即正义或反义策略，将与淀粉合成的相关基因：Wx基因和分支酶基因，通过农杆菌介导法导入不同水稻品种或杂交稻亲本中，获得了一系列转基。
Progress on chorismate mutase gene of plant parasitic nematodes
植物寄生线虫分支酸变位酶基因的研究进展
The specific activities of chorismate mutase and prephenate deh.
结果 T蛋白的表达量达每 1L培养液 2 0 0mg ,细胞裂解液经一次his tag亲和柱色谱 ,纯度达 98% ,分支酸变位酶和预苯酸脱氢酶的比活性分别为 130和 98U·mg-1。
Research on Expression Regulation and Management of Maize Starch Branch Enzyme Genes;
玉米淀粉分支酶基因表达调控的研究
Genetic Diversity of Isozyme of Starch Branching Enzyme and the Effects on the Activity and Amylopectin Content in Wheat Grain;
小麦淀粉分支酶同工酶遗传多样性及对酶活性和支链淀粉含量的影响
Study on Agrobacterium-mediated Genetic Transformation of Indica Rice with SBE Gene;
农杆菌介导的淀粉分支酶基因转化籼稻的研究
Cloning and Functional Analysis of Maize Starch Branching Enzyme Gene SBEⅡb Promoter;
玉米淀粉分支酶SBEⅡb基因启动子的克隆与功能分析
Silencing of Starch Branching Enzyme Genes(sbe Ⅰ and sbe Ⅱb) in Maize by RNA Interference (RNAi);
利用RNAi技术沉默玉米淀粉分支酶基因（sbeⅠ，sbe Ⅱb）表达的研究
Cloning, Sequence Analysis, Construction of Expression Plasmids and Functional Identification of Deltamethrin-resistance Associated Glycogen Branching Enzyme Gene (NYD-GBE) from Culex Pipiens Pallens;
蚊抗药性相关新基因—糖原分支酶基因（NYD-GBE）的克隆和初步鉴定
Characterization, Activity and Purification of Starch-Debranching Enzymes during Wheat Grain Filling Period;
小麦籽粒灌浆过程中淀粉去分支酶的类型、活性及其纯化的研究
Studies on the Activity Differences, Types and Mechanism of Debranching Enzyme in Wheat Grains;
小麦籽粒中淀粉去分支酶的活性差异、类型及作用机制的研究
The Effects of Simultaneously Antisense Gene Targeting of Two Branching Enzymes on the Starch Physiochemical Properties in the Endosperm of Transgenic Rice;
聚合双反义分支酶基因对稻米淀粉理化特性的影响
Studies on Genetic Transformation of Anti-Sense Expression Vector of Starch Branch Enzyme Gene in Maiz;
淀粉分支酶基因反义表达载体在玉米中的遗传转化研究
Transgenic-mediated Knockdown of the Expression of Genes Encoding Starch Branching Enzymes and Its Effects on Grain Quality in Rice (Oryza Sativa L.);
抑制淀粉分支酶类基因表达对稻米品质影响的研究
Construction of Antisense Expression Vector on Corn Starch Branching Enzymes Gene sbe2a and Initial Research of Transgenic Corn
玉米淀粉分支酶sbe2a基因反义载体的构建及其转基因初步研究
Construction of Antisense Bivalent-expression Vectors Containing Cassava SBE Gene
木薯淀粉分支酶基因双价块根特异性反义表达载体的构建
Subcellular Localization and Genetic Polymorphism of Isoform of Starch Branching Enzyme (SBE IIb) in Wheat Grain
小麦籽粒淀粉分支酶同种型SBE IIb的亚细胞定位及遗传多样性
Studies on Cladistic Systemics and EST Isozymes of Unionicolid Mites
蚌螨支序系统分析及其酯酶同工酶多态型研究
Extraction and Spectrum Analysis of Adenosine Phosphorylase of Mycoplasma Mycoides Subsp Capricolum;
丝状支原体山羊亚种腺苷磷酸化酶的提取与酶谱分析
Clinical Analysis of Activity of Serological Myocardial Enzymes in Child with Mycoplasmal Pneumonia;
肺炎支原体肺炎患儿血清心肌酶临床分析
Progress on chorismate mutase gene of plant parasitic nematodes
植物寄生线虫分支酸变位酶基因的研究进展