The effects of fetal bovine serum(FBS) addition time,concentration and different culture systems on the developmental potentiality of bovine parthenogenetic embryos were observed.
试验研究了培养液中血清的添加时间和添加浓度及不同培养体系对黄牛孤雌胚胎体外发育潜力的影响。
The results showed that the culture droplets of 200-500 μL,10 percent of NGS supplemented in all these three media were suitable for development of goat parthenogenetic embryos,the ratio of blastocyst reached 62.
系统地研究了培养液微滴的大小、培养液体系及血清浓度对山羊孤雌胚胎早期体外发育的影响。
The present study was designed to examine the effect of TSA on the maturation of porcine oocytes and development of parthenogenetic embryos in vitro.
检验TSA对猪卵母细胞体外成熟以及孤雌胚胎发育的影响。
Superovulation and parthenogenetic activation in rabbits;
兔卵母细胞的超数排卵与孤雌激活
Parthenogenetic activation of calcium ionophore A23187 and 6-dimethylaminopurine on human oocytes;
应用钙离子载体A23187及6-甲基嘌呤对人卵母细胞进行孤雌激活
Application of sheep sperm extract cytosolic injection in oocytes parthenogenetic activation;
绵羊精子提取物对卵母细胞的孤雌激活作用
Discovery of Parthenogenesis Population of Haemaphysalis longicornis in China and Its Biological Characteristic;
我国长角血蜱孤雌生殖种群的发现和生物学特性的研究
Cloning and Bioinformatics Analysis of a Parthenogenesis-related 30K Lipoprotein Precursor Gene in Bombyx mori;
家蚕孤雌生殖差异脂蛋白30K lipoprotein precursor基因的克隆与生物信息学分析
Bioinformatics Analysis of Heat Shock Related Proteins From Differential Protein Spots Associated With Parthenogenesis in Bombyx mori;
家蚕孤雌生殖差异蛋白——热休克相关蛋白的生物信息学分析
This paper screened and optimized some parameters of nuclear transfer on rabbits,including parthenogenesis,fusion,activation,and development of NT embryos,the nuclear donors were skin epidermal cells of adult rabbits.
以成年家兔上皮细胞为核供体,对兔体细胞核移植中孤雌激活、去核、融合、激活及重构胚发育等环节中的有关参数进行筛选和研究。
The effect of Ca2+ concentration in the electro-activation medium on the parthenogenesis of in vitro maturation(IVM) porcine oocytes was studied in expriment 1.
本文主要研究了激活液中Ca~(2+)浓度对猪体外成熟卵母细胞孤雌激活的影响，以筛选出最佳的Ca~(2+)浓度；研究了猪体外成熟卵母细胞电激活后的体外培养体系，比较了不同培养方式、不同激活液等对猪孤雌激活胚胎体外发育的影响；通过形态观察和Hoechst33342染色，初步探明了猪成熟卵母细胞体外凋亡的规律。
Effect of rare earth on maturation and parthenogenetic activation of mouse oocytes;
稀土对小鼠卵母细胞成熟和孤雌活化的影响
MⅡ stage mouse oocytes were induced to resume meiosis by parthenogenetic activation, and part of them were treated with cytochalasin B and nocodazole, drugs against cytoskeleton.
实验通过孤雌活化诱导小鼠MⅡ期卵减数分裂恢复,采用激光共聚焦显微术检测了减数分裂期间的微管、微丝和核的动态变化,并通过细胞骨架药物处理,以分析微管和微丝在减数分裂事件中的不同作用。
Study of Activation and Parthenogenesis on Bovine Oocytes Matured in vitro for 20～22 h;
体外成熟培养20～22h牛卵母细胞激活及孤雌发育的研究
Porcine oocytes were cultured in NCSU-37,compared effect of in vitro fertilization and parthenogenesis on porcine oocytes in different maturation time (46 h?58 h or 70 h), At 48h after insemination, the cleavage rates in the 46 h group was significantly lower than in 58 h and 70 h groups(P<0.
采用NCSU-37为体外成熟、受精、培养体系,比较不同成熟时间46h、58h和70h的猪卵母细胞对体外受精的影响和孤雌发育。
Effects of electrical pulses combined with chemical activation on the parthenogenetic development of porcine oocytes;
电脉冲与化学激活联合处理对猪卵母细胞孤雌发育的影响
Study on the parthenogenetic development of zona-enclosed or zona-free bovine oocytes;
有无透明带牛卵母细胞孤雌发育研究
Effects of 6dimethylaminopurine (DMAP) on the parthenogenetic development of bovine oocytes were examined in this study.
本研究系统探讨了 6-二甲氨基嘌呤 ( DMAP)对牛卵母细胞孤雌发育的影响。