To further study the biological function and expression of adiponection in vivo, adipoenctin gene knock-out and LacZ gene knock-in mouse model was constructed.
将同源重组的ES细胞克隆注入小鼠囊胚得到嵌合体小鼠,嵌合体小鼠与C57BL/6J小鼠交配产生杂合子小鼠,杂合子间交配获得adiponectin基因剔除LacZ基因敲入纯合子小鼠。
Objective:To modify the original Rosa26 knockin system and introduce the enhanced CA promoter(chicken actin promoter fused with SV40 enhancer).
目的:改造原有的Rosa26基因敲入系统,引入启动能力更强的CA杂合启动子(由鸡actin启动子和SV40增强子融合而成)。
Association of 5-HT-like and substance P-like immunoreactive terminals with mesencephalic trigeminal nucleus neurons in the GAD67-GFP knock-in mouse;
GAD67-GFP基因敲入小鼠5-羟色胺样和P物质样阳性终末与三叉神经中脑核神经元的联系
Colocalization of KCC2 or NKCC1 with GABA in the neurons within the substantia nigra pars reticulata of the GAD67-GFP knock-in mouse
GAD67-GFP基因敲入小鼠黑质网状部神经元GABA与氯离子转运体KCC2和NKCC1的共存
Breeding,reproducing and identifying for aquaporin-3 gene knock-out mice;
水通道蛋白3基因敲除小鼠的饲养繁殖及鉴定
Gene knock-out technology and its application in the research of c-Jun NH2-Terminal Kinase-2
基因敲除技术及其在JNK-2研究中的应用
Construction and characterization of a two-component signal transduction system ciaR gene knock-out mutant of Streptococcus suis serotype 2
猪链球菌2型二元信号转导系统ciaR基因敲除突变株的构建及生物学功能研究
Effect of p53 gene knockout on cell migration;
p53基因敲除对细胞迁移的影响
Analysis of focal cerebral blood flow,ATP content and protein sythesis of L1 gene knockout mice;
L1CAM基因敲除小鼠的脑局部血流量、ATP含量和蛋白合成率分析
Establishment of a βB2 crystallin gene knockout mice model;
βB2晶体蛋白基因敲除小鼠模型的建立
cDNA microarray analysis of transgenic and knock-in prostate cancer mouse models
基因芯片分析比较基因敲入和转基因小鼠前列腺癌模型
Histopathology studies of transgenic and knock-in prostate cancer mouse models
基因敲入和转基因小鼠前列腺癌模型的组织学比较研究
Flow cytometry analysis of transgenic and knock-in prostate cancer mouse models
流式细胞仪分析比较基因敲入和转基因小鼠前列腺癌模型
Effects of zwf2 Gene Knock-in and Disruption on Oxytetracycline Biosynthesis in Streptomyces rimosus M4018
龟裂链霉菌zwf2基因敲入及阻断对土霉素合成的影响
Flow cytometry and cDNA microarray analysis of transgenic and knock-in prostate cancer mouse models
流式细胞仪和基因芯片分析比较基因敲入和转基因小鼠前列腺癌模型
Preclinical Trial Studies of Transgenic and Knock-in Prostate Cancer Mouse Models and the Preparation of Tetramerizing Single Chain Fv Antibody Gene Specific for Human γ-seminoprotein;
转基因和基因敲入小鼠前列腺癌模型的实验研究和多价抗前列腺癌单链抗体的制备
Porcine α-1, 3-galactosyltransferase Gene Knock-out and HLA-G1 Gene Knock-in in Somatic Cells;
猪体细胞α-1，3-半乳糖基转移酶基因的敲除并敲入HLA-G1基因的研究
Constract of Myostatin Knockout Porcine Fetal Fibroblast and Preliminary Study on Myostatin Knockout of Porcine;
MSTN基因敲除猪胚胎成纤维细胞的构建及其基因敲除猪的初步研究
Renal Phenotype of Aging UT-B Knockout Mice and Adult UT-A2/UT-B Knockout Mice
老年UT-B基因敲除和成年UT-A2/UT-B基因敲除鼠肾脏表型研究
Pregnant Phenotype Analysis of AQP3 Null Mice and AQP8 Null Mice
AQP3基因敲除鼠及AQP8基因敲除鼠妊娠相关表型的研究
Application of Cre-LoxP System in The Gene Knockout of Bacillus anthracis and The Knockout of eag Gene
Cre-LoxP系统在炭疽芽胞杆菌基因敲除中的应用及eag基因的敲除
The novel patterns of efficient constituent recognition and quality control for Chinese medicines based on component knock-out & knock-in
基于成分敲除/敲入的中药药效组分辨识与质量控制模式的商建
Human Carcinoma of Esophageal Eca9706 Cell DNA Polymerseβ Gene Targeting;
食管癌细胞Eca9706 DNA聚合酶β基因敲除
Functional Characterization of Fusarium Graminearum PTC1 Gene;
禾谷镰刀菌PTC1基因敲除及功能初探
The Impacts of Kir6.2 Knockout on MPTP-induced Acute Neurotoxicity;
Kir6.2基因敲除对MPTP急性神经毒性的影响
Mechanisms of Cleft Palate Formation in Wnt5a Gene Knockout Mouse;
Wnt5a基因敲除小鼠腭裂发生的机制研究
Functional Characterization of Fusarium Graminearum TEP1 Gene
禾谷镰刀菌TEP1基因敲除及功能研究
Knock Out of Waw in Drosophila and Its Protein Purification
果蝇中waw基因的敲除及其蛋白的纯化