Study on Influence of Technology Parameter on Extraction of RNA from Brewing Yeast by Strong Salt;
影响浓盐法提取啤酒酵母RNA的工艺参数研究
Study on Extracting RNA from Brewing Yeast by Strong Salt Method;
浓盐法提取啤酒酵母中RNA的研究
Extracting RNA from spent brewer's yeast by the new technology rare salt of high temperature of weak base;
高温弱碱稀盐法从啤酒废酵母中提取RNA
Transcription level of trap and RNAⅢ was detected by real time RT-PCR.
方法采用微量板半定量法检测表皮葡萄球菌生物膜表型,二维电泳和高灵敏度的基质辅助激光解吸附飞行时间质谱分析比较表皮葡萄球菌标准株蛋白质表达谱,实时定量逆转录PCR检测trap基因和RNAⅢ的转录水平,CLUSTAL X对表皮葡萄球菌trap基因与金黄色葡萄球菌序列进行分析,Mega软件构建进化树。
Methods Forty-eight samples from patients with ovarian tumor at different clinical stages and 8 from normal ovaries were examined for NM23-H1B mRNA expression by using RT-PCR, northern blot and in situ hybridization.
采用RT-PCR技术、RNA印迹(northern blot)法、原位杂交实验,检测NM23-H1B基因mRNA的表达。
METHODS: The validity and reliability of the results gotten by serial analysis of gene expression method was verified by RT-PCR and Northern blot.
以管家基因磷酸甘油醛脱氢酶(GAPDH)和肌动蛋白(β-actin)的mRNA表达水平为对照,比较了在JT库中表达水平比在WY库中高8倍的JT8标签对应基因的表达。
Methods: Total RNA was isolated from peripheral leukocytes of healthy men and women, AR mRNA was determined by RT PCR and Northern blot.
方法 :采用 RT- PCR和 Northern印迹方法检测健康成年人外周血白细胞 AR m RNA的表达。
Extraction and Yield of Total RNA in Cochleas of Normal Rats and Long-Term Sodium Salicylate Treated Rats;
正常及慢性水杨酸钠作用后大鼠耳蜗总RNA的提取和产量
By using the modified CTAB method, total RNA was isolated from Camellia oleifera leaves rich in such secondary products as polyphones and polymeric carbohydrates, and then mRNA was isolated and purified through double selections using oligo(dT) cellulose.
利用改良的CTAB法,从富含酚类糖类等次生物质油茶叶片中分离出总RNA,并从总RNA中,通过两次过Oligo(dT)柱分离纯化得到mRNA。
The roasted ore produced by the fluidized roasting of high-iron sphalerites characterized by high soluble iron and low soluble zinc and is not applicable in wet metallurgy.
针对高铁闪锌精矿沸腾焙烧产出的焙砂可溶铁含量高、可溶锌率低,不符合湿法冶炼锌工艺要求的问题,借鉴闪锌精矿沸腾焙烧技术操作条件,通过工业化试验研究,寻找炉温、风料比、烟气出口抽力、入炉精矿中铁含量、炉料在炉内停留时间等焙烧技术条件与焙砂中可溶铁、可溶锌等之间的关系,确定适合于高铁闪锌精矿焙烧的技术操作条件。
The concentration of soluble iron extrated from sediments is controlled by three major factors as follows:1.
海洋沉积物可溶铁Fe~(3+)/Fe~(2+)比值,已被海洋地质工作者作为海洋油气化探的一项间接指标应用于东海油气化探中。
The roasted ore produced by the fluidized roasting of high-iron sphalerites characterized by high soluble iron and low soluble zinc and is not applicable in wet metallurgy.
针对高铁闪锌精矿沸腾焙烧产出的焙砂可溶铁含量高、可溶锌率低,不符合湿法冶炼锌工艺要求的问题,借鉴闪锌精矿沸腾焙烧技术操作条件,通过工业化试验研究,寻找炉温、风料比、烟气出口抽力、入炉精矿中铁含量、炉料在炉内停留时间等焙烧技术条件与焙砂中可溶铁、可溶锌等之间的关系,确定适合于高铁闪锌精矿焙烧的技术操作条件。
This paper explores mainly the influence of reduction roasting temperature, time and pulverized coal consumption on content of soluble zinc.
本文主要探讨还原焙烧温度、时间和煤粉用量对可溶锌含量的影响。
Study on the changes of the contents of soluble sugar in the shoot of Guizhou-kuding tea;
贵州苦丁茶新梢中可溶性糖含量变化分析
Relationships between the cotton resistance to the cotton aphid(Aphis gossypii) and the content of soluble sugars;
棉花抗蚜性与可溶性糖含量的关系
Effects of imidacloprid press on the soluble sugar,mineral element and free amino acid in rice plants;
吡虫啉胁迫对水稻可溶性糖、游离氨基酸及钾等矿物元素含量的影响
Bioleaching of soluble phosphate from phosphate rock with acidithiobacillus ferrooxidans mutated with microwave;
微波诱变氧化亚铁硫杆菌浸出磷矿粉中可溶性磷
The function parameters of cyanobacterium were measured including growth curve, total phosphorus contents, polyphosphate contents, and soluble phosphate contents in M.
将铜绿微囊藻(Microcystisaeruginosa)接入不同磷浓度的培养基中进行光照培养,在分析水中溶解性磷浓度的同时,测定铜绿微囊藻的生长曲线和微囊藻中总磷、聚磷、可溶性磷以及糖原含量的变化过程,了解不同外源性磷浓度下铜绿微囊藻的生长和磷代谢过程。