Study on examining class 1 integron of Gram-negative bacteria by colony in situ hybridization;
菌落原位杂交法检测革兰阴性菌Ⅰ类整合子的研究
Objective To study the influential factors on examining Class Ι integron by colony in situ hybridization and optimize the method.
目的探讨菌落原位杂交法检测革兰阴性菌中Ⅰ类整合子的影响因素,并优化该方法。
With the colony hybridization method,the genomic library of Dongxiang wild rice was screened and two candidate clones were obtained.
基于植物的抗性基因具有保守序列,根据已克隆基因的保守序列设计探针,利用菌落杂交的方法,对东乡野生稻大片段基因组文库进行筛选,得到两个候选克隆。