Modulating effect of androgen on ubiquitin ligases in a rat model of skeletal muscle atrophy;
雄激素对骨骼肌萎缩大鼠泛素连接酶的调控作用研究
Development in ligase-mediated techniques for bio-molecular analysis
连接酶介导的生物分子检测技术
In this work, by using specifity, real-time and simplicity of molecular beacons and coenzyme-dependence of DNA ligase, novel detection methods for adenosine triphosphate (ATP), nicotinamide adenine dinucleotide phosphate (NADP), creatine kinase (CK) and protein kinase A (PKA) were developed:1.
本论文基于分子信标技术实时、快速、简便的特点,结合连接酶对辅酶的高度依赖性,以三磷酸腺苷(ATP)、烟酰胺腺嘌呤二核苷酸磷酸(NADP)、肌酸激酶(CK)、蛋白激酶A(PKA)为目标,着眼于发展生物分子检测的新方法,开展了以下四个工作:1。
We describe our construction of a plant expression vector, which contained an UDP-glucose pyrophosphorylase gene and an anti-sense 4-coumarate: CoA ligase gene.
构建了携带紫穗槐尿苷二磷酸葡萄糖焦磷酸化酶(UDP-glucose pyrophosphorylase,UGPase)基因和4-香豆酸:辅酶A连接酶(4-coumarate:coenzyme A ligase,4CL)反义基因的双价基因植物表达载体,用热激法转化至根癌农杆菌LBA4404中,并用制备的农杆菌工程菌进行了烟草转化。
TEB4(MARCH VI)is the homolog of yeast Doa10,an E3 ligase involved in ER-associated degradation.
TEB4(MARCH Ⅳ)是酵母Doa10的同源体,作为E3连接酶参与内质网相关蛋白的降解。
Objective To determine the role of Rho associated kinase (ROK) in contraction of rat femoral arteries after 14 d tail suspension.
目的研究Rho连接激酶 (Rhoassociatedkinase ,ROK)在 1 4d尾部悬吊大鼠股动脉收缩中的作用。
A thermostable DNA ligase gene was identified, and the biochemical and enzymatic properties of the ligase were characterized from JP2 strain which was enriched from geothermally active sites in Papua New Guinea.
对分离自巴布亚新几内亚地热活跃区的一种嗜热耐酸古细菌——JP2菌株中的DNA连接酶基因进行了克隆、表达、纯化,并对其生物化学及酶学特性进行了研究。
DNA ligase from the hyperthermophilic crenarcheon Sulfolobus shibatae (Ssh ligase) was optimally active in the presence of ATP and partially active in the presence of dATP.
极端嗜热古菌——芝田硫化叶菌DNA连接酶(Ssh连接酶) 的最适辅因子为ATP,在dATP存在时,该酶也能表现出较弱的连接活性。
Two genes encoding a NAD+-dependent DNA ligase (LigA) and an ATP-dependent DNA ligase (LigB) were identified in the genome of the extremely radioresistant bacterium.
在耐辐射球菌基因组中有两个基因分别编码DNA连接酶,一个是NAD~+依赖型DNA连接酶(LigA),一个是ATP依赖型DNA连接酶(LigB)。
amino acid RNA ligase
氨基酸rna连接酶
Methods The restriction endonuclease and T4 DNA ligase were used to construct the vector plasmid.
方法载体的构建采用限制性内切酶酶切、4DNA连接酶连接等方法。
Catalytic properties of 338Val-deleted mutant of 4-coumarate:coenzyme A ligase from Populus tomentosa.
毛白杨4-香豆酸:辅酶A连接酶338Val缺失突变分析
Detection of Clamydia trachomatis with ligase chain reaction.
连接酶链反应检测结膜沙眼衣原体
Ufd1 Enhances the Activity of Ubiquitin Ligase gp78;
Ufd1增强泛素连接酶gp78活性的研究
Hrd1 Facilitates Tau Degradation by Ubiquitin-proteasome Pathway;
泛素连接酶Hrd1介导的tau蛋白降解
The Mechanism of E3 Ligase Smurf2 in Liver Diseases
E3连接酶Smurf2在肝病中的作用机制研究
Functional Characteristics on E3 Ubiquitin Ligase SRAR1 in Arabidopsis
拟南芥泛素连接酶SRAR1的功能研究
Regulation of p53 Family Proteins by HECT-type E3 Ubiquitin Ligases
HECT类泛素连接酶对p53家族的调控作用
ligase chain reactio
LCR,连接酶链式反应[两对互补寡核苷酸的连接产物的指数式积累]
Expression,Purification and Enzymatic Characterization of a Thermostable DNA Ligase from Methanocaldococcus jannaschii
詹氏甲烷球菌DNA连接酶在大肠杆菌中的表达纯化及连接活性鉴定
The Effects of Various Recombined Chimeric Cbl Ubiquitin Ligases on the Growth of Tumour Cells;
重组嵌合Cbl泛素连接酶对肿瘤细胞生长的影响
Effects of the Ubiquitin Ligase Pirh2 in Inhibiting Cell Growth of Lung Cancer;
泛素连接酶Pirh2抑制肺癌细胞生长的试验研究
Study on Diagnosis of Neonatal Chlamydia Trachomatis Pneumonia by Ligase Chain Reaction Enzyme Linked Immunoadsorbent Assay
小婴儿沙眼衣原体肺炎的连接酶链反应—酶联免疫吸附法诊断研究
HECT Domain Ubiquitin-protein Ligase Wwp2 Ubiquitinates the Large Subunit of RNA Polymerase II;
HECT家族泛素连接酶Wwp2泛素化修饰RNA聚合酶II大亚基Rpb1
Effect of Fatty Acid-CoA Ligase 4 Inhibitor Triacsin C on the Proliferation and Apoptosis in HepG2 Cells in Vitro;
脂肪酸辅酶A连接酶4抑制剂Triacsin C对HepG2细胞增殖及凋亡的影响
Any of a class of enzymes, including the carboxylases, that catalyze the linkage of two molecules, generally utilizing ATP as the energy donor.
连接酶一种酶，包括羧基酶，能催化两个分子的结合，一般用腺苷三磷酸酶作能量载体
Kinetic Parameters of a Recombinant 4-Coumarate:Coenzyme A Ligase from Populus Tomentosa
重组毛白杨4-香豆酸:辅酶A连接酶催化不同肉桂酸衍生物的酶促动力学研究