A thermostable DNA ligase gene was identified, and the biochemical and enzymatic properties of the ligase were characterized from JP2 strain which was enriched from geothermally active sites in Papua New Guinea.
对分离自巴布亚新几内亚地热活跃区的一种嗜热耐酸古细菌——JP2菌株中的DNA连接酶基因进行了克隆、表达、纯化,并对其生物化学及酶学特性进行了研究。
DNA ligase from the hyperthermophilic crenarcheon Sulfolobus shibatae (Ssh ligase) was optimally active in the presence of ATP and partially active in the presence of dATP.
极端嗜热古菌——芝田硫化叶菌DNA连接酶(Ssh连接酶) 的最适辅因子为ATP,在dATP存在时,该酶也能表现出较弱的连接活性。
Two genes encoding a NAD+-dependent DNA ligase (LigA) and an ATP-dependent DNA ligase (LigB) were identified in the genome of the extremely radioresistant bacterium.
在耐辐射球菌基因组中有两个基因分别编码DNA连接酶,一个是NAD~+依赖型DNA连接酶(LigA),一个是ATP依赖型DNA连接酶(LigB)。
Expression,Purification and Enzymatic Characterization of a Thermostable DNA Ligase from Methanocaldococcus jannaschii
詹氏甲烷球菌DNA连接酶在大肠杆菌中的表达纯化及连接活性鉴定
Methods The restriction endonuclease and T4 DNA ligase were used to construct the vector plasmid.
方法载体的构建采用限制性内切酶酶切、4DNA连接酶连接等方法。
amino acid RNA ligase
氨基酸rna连接酶
V(D)J recombination and DNA non-homologous end joining
V(D)J重组与DNA非同源末端连接
cycle sequencing
循环测序法[例如用嗜热DNA聚合酶和热循环仪直接对双链DNA进行测
Bridge PCR,An Easy Way for Concatemerizing DNA Tags
桥式PCR,一种简易连接DNA标签序列的方法(英文)
Catalytic properties of 338Val-deleted mutant of 4-coumarate:coenzyme A ligase from Populus tomentosa.
毛白杨4-香豆酸:辅酶A连接酶338Val缺失突变分析
Progress in research of PDZ-binding-kinase/T-LAK cell-originated protein kinase
PDZ连接激酶/T-LAK细胞源蛋白激酶研究进展
Detection of Clamydia trachomatis with ligase chain reaction.
连接酶链反应检测结膜沙眼衣原体
Ufd1 Enhances the Activity of Ubiquitin Ligase gp78;
Ufd1增强泛素连接酶gp78活性的研究
Hrd1 Facilitates Tau Degradation by Ubiquitin-proteasome Pathway;
泛素连接酶Hrd1介导的tau蛋白降解
The Mechanism of E3 Ligase Smurf2 in Liver Diseases
E3连接酶Smurf2在肝病中的作用机制研究
Functional Characteristics on E3 Ubiquitin Ligase SRAR1 in Arabidopsis
拟南芥泛素连接酶SRAR1的功能研究
Function and significance of integrin-linked kinase in heart
整合素连接激酶在心脏中的功能意义
Regulation of p53 Family Proteins by HECT-type E3 Ubiquitin Ligases
HECT类泛素连接酶对p53家族的调控作用
This is catalyzed by a transaminase enzyme in conjunction with the coenzyme pyridoxal phosphate.
催化这个反应的酶是氨基转移酶，其上连接有磷酸吡哆醛作为辅酶。
Molecular Docking Studies on the Metallonucleases and Polyamides with Double-strand DNA Molecules
金属核酸酶及寡聚酰胺与双链DNA分子对接模式的理论研究
ligase chain reactio
LCR,连接酶链式反应[两对互补寡核苷酸的连接产物的指数式积累]