Tumor cell-specific gene transfer with retroviral vectors displaying single-chain antibody;
单链抗体-包膜蛋白嵌合型单嗜性逆[转]录病毒构建及靶向感染肿瘤细胞的研究(英文)
Methods Recombinant retroviral vector pLNCX/iNOS was transferred into the amphotropic packaging cell line PA317 by lipofectamine.
方法 用PCR技术从 pCMV/iNOS质粒中扩增出iNOS全长cDNA ,与逆[转]录病毒pLNCX构建重组质粒 pLNCX/iNOS ;通过脂质体介导把重组质粒转染入包装细胞PA3 17中 ,经G418筛选出抗性克隆。
Methods Full length cDNA of iNOS were amplified by PCR from pCMV/iNOS vector with retroviral vectors to construct pLNCXiNOS.
方法 :用PCR技术从pCMV/iNOS质粒中扩增出iNOS全长cDNA ,与逆[转]录病毒pLNCX构建重组质粒pLNCX/iNOS ;通过脂质体介导把重组质粒转染入包装细胞PA3 17中 ,经G418筛选出抗性克隆。
Construction and identification of recombinant defective retroviral vector with mutant human proinsulin gene and stable virus-producing cell lines;
人胰岛素原突变体重组逆[转]录病毒及稳定包装细胞系的构建和鉴定
Antitumor Effected by Retroviral Expressing wtp53 Gene Driven by hTERT Promoter;
hTERT启动子调控p53基因表达逆[转]录病毒的抗肿瘤作用
The titer of retroviral was estimated by Hela.
目的 建立产逆[转]录病毒的PA31 7/HGSTπ细胞株 ,为进一步把耐药基因GSTπ导入造血干细胞以逆转卵巢癌化疗药物对骨髓的毒副作用的研究奠定基础。
Retrovirus-mediated expression of enhanced green fluorescent protein in rat bone marrow stem cells;
逆[转]录病毒介导增强型绿色荧光蛋白基因在大鼠骨髓间充质干细胞中的表达
Research on recombinant retroviruses entegrated with anti-CD20 scFv/CD80 /CD28/ ζ gene;
重组anti-CD20 scFv/CD80/CD28/ζ非复制型逆[转]录病毒的构建
The culture of retrovirus package cell and the package process;
逆[转]录病毒载体包装细胞的培养及包装过程(英文)
Construction of Foxp3 retroviral vector and its suppressive function on the induced CD4~+CD25~+Foxp3~+ T cells;
Foxp3逆[转]录病毒载体的构建及其诱导的CD4~+CD25~+Foxp3~+ T细胞免疫抑制功能研究
Construction and expression of recombinant retroviral vectors expressing siRNA for MDR1 gene;
MDR1基因RNAi逆[转]录病毒载体的构建及表达
Apoptosis of pancreatic carcinoma mediated by a retroviral vector containing antisense human telomerase gene;
逆[转]录病毒载体介导的端粒酶抑制基因促胰腺癌细胞凋亡
Construction of retrovirus expressing CTGF siRNA and its steady packaging cell line mediated by PRS retrovirus vector;
PRS-CTGF-SiRNA逆[转]录病毒载体及包装细胞株的构建
Construction of recombinant retrovirus vector bearing antisense AFP enhancer/TK gene and its hepatocyte-specific expression;
反义AFP增强子/TK基因重组逆[转]录病毒载体的构建及在肝癌细胞中的专一性表达
Report on design and construction of recombinant retrovirus vector expressing siRNA for survivin gene knockdown;
survivin基因RNAi逆[转]录病毒载体设计与构建方法实验研究
Comparison of two methods of the recombinant retrovirus;
两种重组逆[转]录病毒浓缩方法的比较
Development of RT-nested PCR for detection of antisense RNA recombinant retrovirus;
反义RNA重组逆[转]录病毒逆转录套式PCR检测方法的建立
Study on in vitro concentration of recombinant retrovirus;
重组逆[转]录病毒浓缩方法研究
Methods The cultured ECV304 was transduced with tPA gene using VSV-G/MuLV.
方法利用携带tPA基因的假型逆[转]录病毒转导血管内皮细胞株ECV304,用G418筛选法观察基因是否成功转导,采用发色底物显色法检测转基因ECV304细胞培养上清液中tPA纤溶活性变化。