Cloning and Sequencing of Tumor Suppressor Gene LKB1/STK11 Associated with Peutz-Jeghers Syndrome by RT-PCR;
PJ综合症相关抑癌基因LKB1/STK11的逆转录PCR法克隆及序列分析
Methods Expression of PLC-epsilon in bladder tissues of 27 patients with TCCB and 12 normal bladder tissues was detected by RT-PCR,and its relationship with clinicopathological characteristics of tumor was analyzed.
方法采用逆转录PCR(RT-PCR)方法检测27例膀胱移行细胞癌和12例正常膀胱对照组中PLCε mRNA表达,分析其与膀胱病理学特征的关系。
Me thods Total RNA was isolated from rat brain tissue, and the coding region of DNA polymerase β was amplified by reverse transcriptase - polymerase chain reaction (RT-PCR).
方法提取大鼠脑组织总RNA,用逆转录PCR扩增DNA多聚酶β(POLB)的编码区,与T载体连接,作DNA测序后,酶切POLB基因并连入腺病毒穿梭质粒pAdTrack-CMV,电转至含腺病毒pAdeasy骨架质粒的大肠杆菌BJ5183。
Methods The expression of SSTR subtype 1～5 mRNA was determined by reverse transcription polymerase chain reaction method in 30 cases of pancreatic cancer and in 12 cases of normal pancreas tissues.
方法应用逆转录PCR(RT-PCR)检测30例胰腺癌组织和12例正常胰腺组织生长抑素受体亚型mRNA的表达。
The expression of multidrug resistance(mdr 1) gene was tested by RT-PCR and Western blot.
方法采用MTT法检测X射线照射后CNE-1细胞对顺铂(cD-DP)的耐药性;采用逆转录-PCR(RT-PCR)和蛋白免疫印迹(W estern b lot)方法,检测照射后CNE-1细胞mdrl基因的表达。