pylori with balanced lethal system MethodsThe gene of urease B subunit of H pylori was amplified and recombined with a vector(asd +) The recombinant was transformated into attenuated salmonella typhimurium The expression of urease B subunit was analysed by SDS PAGE and western blot, also the stability of vaccine strains was investigated by generation culture in vitro ResultsAn a.
目的采用平衡致死系统构建表达幽门螺杆菌尿素酶 B亚单位的减毒沙门氏菌工程菌株。
In the current work, the fusion gene including somatostatin (SS) and the hepatitis B surface antigen gene was cloned into a balanced lethal system plasmid (pYA3493), and then transformed into asd? attenuated Salmonella choleraesuis C500 strain, the positive transformant without antibiotic resistance gene was confirmed by restriction analysis and DNA sequencing, designated as pYA-SS.
将生长抑素(SS)与乙肝表面抗原(S)融合基因插入平衡致死系统原核表达质粒pYA3493中,转化至缺失asd基因的减毒猪霍乱沙门氏菌C500,经酶切、测序筛选得到非抗性的目的克隆,命名为pYA-SS。
The construction and application of chromsome-plsmid balanced-lethal system;
染色体-质粒平衡致死系统的构建及应用
Research of sex determination is the basic foundation for insect sex control which can be efficiently controlled using balanced sex linked lethal genes, sex specific expression of d.
研究昆虫的性别决定是人们进行昆虫的性别控制的基础 ,随着基因工程的发展 ,人们可以利用性连锁平衡致死基因、性别特异性致死基因、杀雄微生物等多字方法对昆虫的性别进行控制。
Construction of the Chromosome-Plasmid Balanced-Lethal System Based on E.coli Thymidylate Synthase Gene;
基于thyA基因的染色体—质粒平衡致死系统的构建
A Cross Breeding Method for Sex-linked Balanced Lethal Line of Silkworm
家蚕性连锁平衡致死系的杂交改良方法
Study on the Breeding of Male Silkworm Varieties Using Lethal Lines Balanced on Silkworm Sex Chromosome
利用性连锁平衡致死系进行雄蚕品种选育的方法研究
Functional Display of the F18 E.coli Adhesin on the Bacterial Surface and Construction of the Chromosome-plasmid Balanced-lethal System;
F18大肠杆菌黏附素菌体表面功能性展呈及其染色体—质粒平衡致死系统的构建
The major factors causing death are electrolyte imbalance and dehydration, leading to cardiac arrest.
引起死亡的主要原因是电解质的不平衡和脱水,导致心脏停搏。
inactivity resulting from a static balance between opposing forces.
由于静态平衡而导致的没有运动。
Secular disequilibrium of uranium can lead to erroneous results.
铀的永久不平衡会导致错误的结果。
make level, square, balanced, or concentric.
使达标,一致,平衡或同中心的。
A Consistency Axiomatization of Correlatedly Bayesian Equilibrium
相关贝叶斯平衡的一致性公理化(英文)
A Consistency Axiomatization of Coalition-proof Correlated Equilibria;
防结盟相关平衡的一致性公理化(英文)
Statistical Analysis for Comparing the Mean Over Frequency of Embryonic Deaths in Dominant Lethal Assay;
医学显性致死试验平均死胎率比较的统计分析
Trying to make ends meet, you are a slave to the money then you die.
试着使收支平衡,你成为金钱的奴隶直到死去.
The man used his weight to counterbalance the load other
那人用身体的重量使负荷保持平衡不致往下滑。
Keywords: supercritical fluid, consistency test, theoretical model, phase equilibria.
关键词:超临界流体、一致性测试、理论模式、相平衡。
Many stat changes fixing inconsistency errors and some balancing problems.
修正一些平衡性及导致错误产生的问题。
Such herbs will strive to more greatly balance the energy flow.
这类草本将致力于使能量流获得更大平衡。
Continuous Darkness Causes Balance Disturbed Effect on In-vivo Elements of Rats;
持续黑暗所致大鼠体内元素平衡紊乱的影响
The Imbalance and Wind-Induced Vibration of the Rotating Beam in Cantilever State under Construction;
转体梁施工中的不平衡问题及风致振动研究