To clone the differential expressional genes between the pre-metamorphosis(17 day post hatching,DPH) and pro-metamorphosis stage(23 DPH),we used suppression subtractive hybridization(SSH) to construct a cDNA library of pro-metamorphosis flounder Paralichthys olivaceus,when the RNA from pre-metamorphosis larvae was used as the driver.
为了克隆变态早期牙鲆头部差异表达的基因,采用抑制差减杂交法,以变态前的仔鱼头部表达的RNA作为驱动RNA,建立了牙鲆变态早期的差减cDNA文库,并利用相对定量RT-PCR对其进行了筛选。