To investigate the spatio-temporal expression of hypoxia-inducible factor-1 (HIF-1) during murine neurulation and explore the role of HIF-1 in the embryonic development of the nervous system.
为研究低氧诱导因子-1(HIF-1)在小鼠胚胎神经胚形成阶段的时空表达规律,探讨HIF-1在胚胎神经系统发生和发育过程中的作用。
Objective To observe the expression of STAT3 in the process of neurulation, the differentiation and migration of the progenitor cell of mouse.
方法 采用全胚胎原位杂交技术和免疫组织化学技术,观察STAT3在神经胚形成和神经上皮分化迁移阶段的表达。
Purpose: 1 To investigate the role of TGF-β1 in in vitro culture of mouse embryo and embryo implantation by comparing the development status and implantation rate after blastocyste transfer between embryos cultured in M16 and M16 containing different level of TGF-β1.
方法：1、比较小鼠2-细胞期胚胎在不同胚胎培养组(M16组、M16+TGF-β1 1ng／ml、M16+TGF-β1 10ng／ml和M16+TGF-β1 50ng／ml组)培养第68及92小时早期囊胚、扩张囊胚和孵出囊胚形成率：并于胚胎培养第68小时，将M16组和最适宜TGF-β1浓度组扩张囊胚进行胚胎移植，比较两组移植小鼠胚胎种植率。