The isolated plasmid DNA was quantified by spectrophotometric measurement,and evaluated by the electrophoresis of plasmid DNA in an agarose gel,the restriction enzyme digestion,and the transfection into eukaryotic cells.
方法将含有目的质粒的菌株扩增后,运用改良后的碱裂解法进行质粒DNA小量提取,微量核酸仪测定提取质粒DNA的产量及纯度;采用琼脂糖凝胶电泳、内切酶酶切及质粒转染真核细胞鉴定提取质粒DNA的质量及转染效率。
Contribution of Conservative Cleavage Motif to the Proteolytic Cleavage Within Carboxyl Terminal Domain of Rodent Muc3;
大鼠粘蛋白rMuc3酶切保守基序对其蛋白酶切的影响的研究
Contribution of conservative cleavage motif to proteolytic cleavage within carboxyl terminal domain of rodent Muc3
大鼠黏蛋白rMuc3酶切保守基序对其蛋白酶切的影响
Efficiencies of cleavage inboth cases were higher than those by liquid-phase dilution method.
对两种融合表达的药物蛋白尝试了固相柱上酶切的方法,即Trx-r-PA、GST-IL-11融合蛋白分别在ETI-SepharoseFF、GST-Agarose亲和柱上酶切,酶切效率均高于液相酶切法。
NGF by enzyme digestion was inserted into an eukaryotic expression vector,PcDNA 3 with T4 ligase.
方法 将质粒PGEM β NGF进行酶切获得 β NGF基因片段 ,用T4连接酶将其插入真核表达载体PcDNA3;以T7、P2为引物进行PCR ,论证插入片段的方向 ;经测序和酶切对插入片段进行分析和进一步鉴定。
The G → A mutation created a Hha Ⅰ enzyme digestion position and the frequency was studied by asymmetric PCR-SSCP and enzyme digestion in 116 Luxi cattle and 75 Holstein cows and got the same results.
利用不对称PCR-SSCP技术,在鲁西黄牛和荷斯坦奶牛SMAD4基因的3’端非翻译区找到了1个碱基T插入突变位点和1个G→A突变位点,其中G→A突变产生了一个Hha Ⅰ酶的酶切位点。
Preparation of highly purified vector DNA is affected by a series of factors including digestion of restriction enzyme and dephosphorylation of linearized vector DNA.
制备高质量的BAC载体DNA受到包括酶切、脱磷等诸多因素的影响。
The Genomic DNA samples extracted by three methods were tested by agarose gel elctrophresis and restriction endonucleas digestion,Based on the comparative analysis of yield and quality of the DNA samples by three methods,the result showed that DNA yield of CTAB method was higher than that of SDS method and.
并通过琼脂糖凝胶电泳、限制性内切酶酶切对3种方法提取的DNA样品进行检测。
circular restriction map
环形限制（酶切）图（谱）
The Application of the Artificial Neural Network to the Study of Proteasome Cleavage;
ANN方法在蛋白酶体酶切研究中的应用
Methods The restriction endonuclease and T4 DNA ligase were used to construct the vector plasmid.
方法载体的构建采用限制性内切酶酶切、4DNA连接酶连接等方法。
Contribution of Conservative Cleavage Motif to the Proteolytic Cleavage Within Carboxyl Terminal Domain of Rodent Muc3;
大鼠粘蛋白rMuc3酶切保守基序对其蛋白酶切的影响的研究
Contribution of conservative cleavage motif to proteolytic cleavage within carboxyl terminal domain of rodent Muc3
大鼠黏蛋白rMuc3酶切保守基序对其蛋白酶切的影响
complete digestion
完全消化[多指限制酶切反应]
A study of GC-subtype polymorphism in Han population of Wenzhou city using mismatch primer-induced restriction sites
错配引物诱导酶切技术检测GC多态性
Any of several enzymes, such as endonucleases and exonucleases, that hydrolize nucleic acids.
核酸酶水解核酸的酶，如核酸内切酶和核酸外切酶
AP endonuclease
脱嘌呤嘧啶内切核酸酶
Characterization and Application of the Recombinant Truncated Endoglucanase Without Cellulose Binding Domain (CBD) Expressed in Pachia Pastoris;
重组内切纤维素酶酶学性能及其应用研究
Preliminary Study on Immobilization for Endo-Chitosanase and Its Properties
内切壳聚糖酶的固定化及其酶学性质初步研究
Some Enzymologic Characteristics of Garlic Fructan Exohydrolase
大蒜果聚糖外切酶的一些酶学特征研究
Secondly, special enzymes called extinction enzymes are used to cut the DNA at specific places.
第二，利用被叫做限制性内切酶的特殊的酶在特定地方切断DNA。
Optimization and Kinetics of Enzymatic Hydrolysis of Aspergillus ficuum Endoinulinase
Aspergillus ficuum内切菊粉酶的酶解条件优化及酶解动力学研究
ECU (Endo Cellulase Units)
内切纤维素酶活力的单位
The application of thronbin ice in tonsillectomy
凝血酶冰块在扁桃体挤切术后的应用
Studies on the Quality and Enzymatic Browning Mechanism of Freshcut Artemisia Selengensis;
鲜切芦蒿的品质和酶促褐变机理研究
The Studies on the Endo-β-1,4-glucanases from the Mollusca, Ampullaria Crosseans;
福寿螺（Ampullaria crossean）内切-β-1，4葡聚糖酶的研究