Methods A synonymous mutation and a hybrophobic polypeptide linker(Gly_(4)Ser)_(3) were used to splice three different gene fragments of β2m and HLA-A2 extracellular region subunits by overlap-PCR for constructing single chain β2m-HLA-A2-BSP fusion gene in vitro.
方法应用重叠延伸PCR(overlap-PCR)对编码HLA-A2的cDNA序列进行一个碱基的同义突变,并将β2m和HLA-A2胞外段亚单位两段编码序列的cDNA通过一疏水性多肽接头(Gly4Ser)3的DNA序列进行前后拼接,并利用引物所带的BSP编码序列,构建单链β2m-HLA-A2-BSP融合基因,将测序正确的β2m-HLA-A2-BSP融合基因插入原核表达载体pET-22b中,转化BL21(DE3)细菌,SDS-PAGE检测其表达,融合蛋白于体外抗原肽存在条件下稀释复性后由Western blot鉴定其空间构象。
Unit Root Spurious Test for Time Series with Structural Change;
结构突变时间序列单位根的“伪检验”
It is thought to take about a year for this mutation to happen.
发生回复突变的时间一般约需1年。
Effect of Sudden Change of Illumination Time on Performance of Chicken
光照时间突变对鸡群生产性能的影响
not continuous in space, time, or sequence or varying abruptly.
在空间、时间或者序列上不连贯或者突然变化。
The Study on Relativity of the Time of Traumatic Diskal Hernia and the Change of CT Rate;
外伤性椎间盘突出时间与CT值改变的相关性
Study on the Geothermal Indirect Central Heating Technology Considering Sudden Climate Change;
冬季气候突变时地热间接供暖的应变技术研究
The research of dynamic structure abrupt change of nonlinear time series
非线性时间序列的动力结构突变检测的研究
Study on wavelet detection of change point of stock exchange index time series;
股指时间序列突变点小波检测研究(英文)
Heuristic segmentation method for change-point analysis of hydrological time series
水文时间序列突变点分析的启发式分割方法
Bi-directional Changes in Dendritic Spatial Summation Accompanying Long-term Synaptic Modifications;
长时程突触增强或抑制所伴随的树突空间整合特性的双向变化
Study of Deducing Time of Traumatic Intervertebral Disc Herniation Applying CT Value and Variation in Morphology;
应用CT值及形态学变化推断外伤性椎间盘突出时间的研究
Analysis of Oilseed Rape Flowering Time Variation and Cloning of Arabidopsis Late Flowering Time Gene;
油菜开花时间变异分析和拟南芥晚开花突变体鉴定与基因克隆
Suddenly, everything was dark.
突然间，一切都变暗了，
About halfway through, this story has a major turnabout.
大约在中间部分的时候，这个故事突然有一个大大的转变。
His heart missed a beat and his face fell. He could not think what to say for a moment.
冯云卿的心突地一跳，脸上变色，暂时之间回答不来。
but suddenly there was an explosion of culture that changed the music and fashion worlds forever.
但是突然间文化的爆发永远改变了音乐和时尚的世界。
The expression Changes of the synaptophysin in the hippocampal formation during the spatial discriminative learning and memory in rats;
大鼠在空间辨别性学习记忆时海马结构内突触素表达的变化
The Research on Bayesian Measure Model of Change Points in Shanghai and Shenzhen Stock Index Time Series;
沪深两市股指时间序列突变点贝叶斯检测模型研究