Objective To construct the prokaryotic expression plasmid expressing woodchuck hepatitis virus core antigen(WHcAg) and prepare polyclonal antibodies.
目的构建土拨鼠肝炎病毒核心蛋白质粒并进行原核表达、抗体制备。
【Method】 The woodchuck hepatitis virus posttranscriptional regulatory element(WPRE) was amplified by polymerase chain reaction(PCR)from the plasmid containing WPRE.
【方法】将PCR扩增土拨鼠肝炎病毒(Woodchuck Hepatitis Virus,WHV)转录后调控元件(WPRE),克隆于pGM-T载体获得重组质粒pGM-WPRE,利用NotⅠ酶将WPRE亚克隆入pcPPTPRE(+),筛选WPRE正向连接的重组质粒pcPPTWPRE,采用磷酸钙法分别将pcPPTWPRE和pcPPTPRE(+)转染HEK293细胞和DF-1细胞,利用荧光显微镜观察EGFP蛋白的表达,利用流式细胞仪检测转染细胞中EGFP阳性细胞的表达率。
reddish_brown North American marmot.
北美洲红褐色的土拨鼠。
true squirrels; ground squirrels; marmots; chipmunks; flying squirrels; spermophiles.
松鼠；黄鼠；土拨鼠；花栗鼠；美洲飞鼠；欧黄鼠。
In November the ground hogs all hole up for the winter.
11月份土拨鼠都入洞冬眠。
My honey and me all like marmot so much, so we baught one which could make the real sound, very cute!
老公和我都非常喜欢土拨鼠，所以我们买了一只连同叫声都仿真的土拨鼠玩具，非常可爱！
heavy-bodied yellowish-brown marmot of rocky areas of western North America.
北美洲西部多岩地区体重的黄褐色土拨鼠。
An Expression of Mouse in a post-modern way;
姿意的狂欢——对《土拨鼠》的后现代主义解读
The Construction and Eukaryotic Expression of the DNA Vaccine Encoding the Fusion of Woodchuck CTLA-4 Extracellular Segment and Hepatitis B Virus Core Protein and Mice Immunization;
土拨鼠CTLA-4胞外段与HBc融合DNA疫苗的构建、真核表达和小鼠免疫
To survive as a lone marmot, you have to be tough.
一只独立的土拨鼠要想能生存下去，它必须要能经受住风风雨雨。
In Heiligenblut, almost all the souvenir shops are selling the marmot toys.
在海立根布鲁特，几乎每家纪念品商店都出售土拨鼠玩具。
In last year's Groundhog survey, the consensus estimate was for net written premium growth of3.8 percent(1).
在去年的土拨鼠调查中，大多数人对净保费的估计值是3.8个百分点。
On the way to the Tappenkarsee, we heard the marmot's voice, but could not see them.
在去塔彭卡湖的路上，我们听到土拨鼠的叫声，但是总看不到它们。
A big marmot toy sitting on the bike, looked so funny!
一只巨大的土拨鼠玩具坐在自行车后座上，看起来真逗！
Characterization of the Expression of LDH Isozymes Genes During Non-hibernation Himalayan Marmot
喜马拉雅土拨鼠非冬眠期乳酸脱氢酶同工酶基因表达特征
A groundhog would hog all the ground he could hog, if a groundhog could hog ground.
一个土拨鼠能够拱起它能拱起的所有土地，如果一个它能够拱起土地。
The mere fact of wandering and surviving might therefore be a good indication of genetic fitness.
因此，一只四处流浪且生存下来的土拨鼠就很好地代表了它拥有绝好的遗传基因。
under the name of Major Brack you run thoroughbreds ridden by tiny urchins not larger than marmots;
您以布莱克参谋先生的名义去参加赛马，派去了纯种的骏马和并不比土拨鼠大多少的骑师；
In the hut, we found a marmot specimen, I liked it so much, then took a picture for it.
在小木屋里，我们发现有只土拨鼠标本，我很喜欢它，于是就给它拍了张像片。
Whether there are any lessons in this for human adulterers remains to be seen.
而土拨鼠的这种情况对人类的奸夫淫妇们而言是否有所教训还有待进一步的发现。