[Methods] Using T cruzi amastin DNA sequence as a reference,computer search was done on GenBank and dbEST databases by using BLAST path.
[目的 ]克隆硕大利什曼原虫无鞭毛体蛋白 (amastin)的编码基因序列。
Amastigotes, epimastigotes and trypomastigotes were produced by schizogony or pseudocyst in leech s crops.
虫体在绿蛙蛭嗉囊的上皮细胞内进行裂配生殖或假包囊，产生许多无鞭体、前鞭体和锥虫体等不同发育时期。
Cloning of Amastin Gene of Leishmania donovani Isolates from Sichuan Province and its Expression in Eucaryotic System;
杜氏利什曼原虫四川分离株无鞭毛体蛋白基因的克隆及真核表达
Objective To construct eukaryotic expression recombinant plasmid of amastin gene of Leishmania donovani.
方法提取杜氏利什曼原虫基因组DNA进行PCR扩增,将扩增出的无鞭毛体蛋白基因片段导入质粒载体pcDNA3。
AIM:To construct recombinant eukaryotic expression plasmid of amastin gene of Leishmania Donovani and detect expression of the gene in NIH3T3 cells.
将扩增的无鞭毛体蛋白基因片段导入质粒pcDNA3。