Methods:A total of 102 suspected cases of early syphilis patients and 30 patients with non-secretory syphilis were detected by real-time quantitative polymerase chain reaction(FQ-PCR),treponema pallidum hemagglutination test(TPHA),treponema pallidum enzyme-linked immunosorbent assay(TP-ELISA) serum rapid reaction(RPR) and serum treponema pallidum(TP) detection.
方法:采用实时荧光定量聚合酶链反应(FQ-PCR)、梅毒螺旋体血凝试验(TPHA)、梅毒螺旋体酶联免疫吸附试验(TP-ELISA)、快速血清反应(RPR)法对102例早期疑似梅毒患者、30例非梅毒患者分泌物、血清进行梅毒螺旋体(TP)检测,比较4种检测法灵敏度及特异性。
Recent studies prove thatRhoA/Rho kinase regulats smooth muscle specific actin dynamics by activating serum response factor (SRF)-dependent transcripti.
应用免疫组化和Westernblot方法分析去血清前后HUVECs血清反应因子(serumresponsefactor,SRF)的表达及定位,Rhodamine-Phalloidine染色观察F-actin动态变化。