Objective To establish a new method for single nucleotide polymorphism (SNP) typing based on allele specific PCR: fragment length discrepant allele specific PCR (FLDAS-PCR), and study the influence on specific extension by introducing a mismatch at the third or fourth 3′-terminal base of allele specific primers.
目的建立一种基于等位基因特异性PCR原理的改良SNP分型新方法:片段长度差异等位基因特异性PCR,并考察特异性引物的3'端第3位、第4位碱基错配对特异性延伸的影响。
Based on the singular isoparametric element and fracture mechanics, this paper analyzes and discusses the problem about the singular crack in pavement structure under three types of load.
应用奇异等参元及断裂力学理论 ,对 3种荷载简化模式下路面单裂缝问题进行了数值分析 。
RAPID IDENTIFICATION OF BENOMYL AND DIETHOTHFENCARB RESISTANCE STRAINS OF BOTRYTIS CINEREA USING AN ALLELE-SPECIFIC PCR (ASP);
应用特异等位PCR快速鉴定灰霉病菌抗苯莱特与乙霉威菌株