This study was aimed to investigate the effect of plasmid-mediated RNAi targeting hTERT on blocking hTERT gene and inhibiting telomerase activity in leukemia cell line K562.
本研究探讨质粒载体介导的RNAi靶向hTERT对白血病细胞株K562 hTERT基因封闭、端粒酶活性抑制的作用。
Construction and identification of plasmid vector of RNA interference of transforming growth factor-β1 gene
转化生长因子-β1基因RNAi质粒载体的构建与鉴定
A new plasmid vector pMD-Fib-IE-EGFP which contains enhanced green fluorescent protein gene as reportor gene was constructed by subtitute EGFP for GFP in the original plasmid pG350.
以含家蚕丝心蛋白重链基因同源片段的质粒pG35 0为出发质粒 ,插入增强型绿色荧光蛋白 (EGFP)基因 ,构建成以EGFP为报告基因的同源重组质粒载体 pMD Fib IE EGFP ,通过PCR及酶切鉴定表明EGFP以正确的方式插入到原始质粒中 ,通过脂质体介导法转染胃癌细胞能发出很强的绿色荧光 ,表明该质粒能够在真核细胞中表达 ,为进一步建立完善的家蚕转基因系统平台提供基因材
Effective Production and Ex vivo Evaluation of Plasmid Vector Intended for Human Cancer Gene TherapyABSTRCTThe effective production of plasmid vector intended for gene therapy was investigated systematically.
本文较系统地研究了用于肿瘤基因治疗的质粒载体的高效制备工艺。
The target blunt-ended DNA was cloned into plasmid vector pUC18 which was previously digested with Sma I and dephosphorylated with calf alkaline phosphatase.
6~3 kb之间的DNA片段用T4 DNA聚合酶补平,再与SmaI酶切消化并经去磷酸化处理的质粒载体pUC18连接,转化至DH10B大肠杆菌(Escherichia coli)的感受态细胞中。
Then use T4 ligase to connect recycled DNA and pUC18 carrier.
以不吸水链霉菌梧州新亚种为材料,提取的总基因组经Sau3AI不完全酶切,回收3~9 kb DNA酶切片段,用T4连接酶将回收片段按一定比例与经BamHI酶切、南极热敏磷酸酶去磷酸化处理后的pUC18质粒载体连接,热激法转化受体菌E。
Construction and Evaluation of Recombinant Plasmid of Livin shRNA
Livin shRNA质粒载体的构建及评价
Influence of Bifidobacterium plasmid polymerase on stability of plasmid vector
双歧杆菌质粒聚合酶基因对质粒载体稳定性的影响
The Construction of Shuttle Plasmid Vector of Streptomyces and E. colt
链霉菌和大肠杆菌穿梭质粒载体的构建
The Expression of NgR mRNA in the Optic Nerve and the Construction of NgR Specific siRNA Encoding Vector;
视神经NgR mRNA的表达及其siRNA表达质粒载体的构建
Construction and Identification of pExpress-1 Expression Vector for rHSG
rHSG/pExpress-1重组质粒载体的构建及序列分析
Experimental Study on siRNA Screening of Cathepsin K Gene and Construction of Its Plasmid Vector
Cathepsin K基因siRNA的筛选及其质粒载体的构建
Cloning and expression of 3α-hydroxysteroid dehydrogenase gene in E.coli
3α-羟类固醇脱氢酶基因的质粒载体构建和表达
Construction of Plasmid Expression Vector for Specific Peptide of the Rubella Virus E1 Gene
风疹病毒E1特异肽段的重组质粒载体构建
Construction and identification of plasmid vector of RNA interference of transforming growth factor-β1 gene
转化生长因子-β1基因RNAi质粒载体的构建与鉴定
Study on Predominant Intestinal Microbe Flora of A.germari Larvae and Construction of the Recombined Clone Vector Containing Gene Cry3A;
桑粒肩天牛幼虫肠道优势菌群研究及Cry3A重组质粒载体构建
Granular activated carbon from coal for catalyst carrier
GB/T7701.3-1997触媒载体用煤质颗粒活性炭
Inhibitory effect of a HBV vector plasmid expressing A3C on HBV replication
表达A3C的HBV载体质粒抑制HBV的研究
The Experimental Study of the Plasmid as a Vector of rhBMP Gene Transfer Therapy Stimulating Reparative Dendtinogenesis;
质粒为载体BMP基因转移促进修复性牙本质形成
The Preparation and Properties Research of Drug Loaded Solid Lipid Nanoparticles;
载药固体脂质纳米粒的制备及性质研究
Research on the Microparticles Composed of Chitosan and Liposomes for Drug Carriers
壳聚糖脂质体复合微粒作为药物载体的研究
Research advances on Solid lipid nanoparticles as new drug carrier
固体脂质纳米粒给药新载体的研究进展
The Studies on Constructing Expression Vector of Nematode Resistance Gene and Genetic Transformation
抗线虫基因表达载体质粒构建和遗传转化研究
Construction and Identification of Rat NeuroD Gene Eukaryotic Expression Vector;
大鼠NeuroD真核质粒表达载体的构建和鉴定