Advance in human artificial chromosome research;
人类人工染色体(HACs)研究进展
Thereinto, bacterial artificial chromosome(BAC) library is the most widely used one at present due to its higher transformation efficiency, lower chimerism level, easier insert fragments isolation and larger capability.
本文以大熊猫的成纤维细胞和肝组织细胞为材料,以pBeloBAC11为载体,构建了大熊猫基因组的细菌人工染色体DNA文库。
Objective:To modify human α-lactalbumin yeast artificial chromosome(HLA-YAC)and to use it as vectors for analysis of gene expression and regulatory elements of α-lactalbumin in vivo in the future.
目的?对人乳白蛋白酵母人工染色体(HLA-YAC)进行定点改造,以便进一步利用该载体作与基因表达调控相关的 研究。
Methods Three clones of yeast artificial chromosomes (YAC) related to bcr gene were examined by FISH in combination with Alu-PCR to identify metaphases peripheral lymphocytes from normal individuals and metaphases bone marrow cells from CML patients with chromosome t (9; 22).
方法应用Ａｌｕ－ＰＣＲ和荧光原位杂交（ＦＩＳＨ） 技术，对３个酵母人工染色体（ＹＡＣ）候选克隆进行鉴定，制备ＤＮＡ探针，并经正常人外周血淋巴细胞和慢性髓细胞性 白血病患者骨髓细胞筛选。
The aim of this study was to construct the complete genome of Marek s disease virus serotype 814 strain as an infectious bacterial artificial chromosome (BAC).
为构建全基因组鸡马立克氏病病毒814株感染性细菌人工染色体(bacterial artificial chromosome,BAC),首先通过构建表达Eco-gpt(xanthine-guanine phosphoribosyl transferase,XGPRT,gpt)的哺乳动物细胞基因转移遗传选择标记(1-3 kb)和带有细菌人工染色体的基本功能基因序列的鸡马立克氏病病毒重组病毒转移载体pUAB-gpt-BAC11,将重组病毒转移载体与鸡马立克氏病病毒细胞总DNA共转染鸡胚成纤维细胞,在选择培养基中经过8轮加压筛选,获得并纯化重组病毒;将重组病毒细胞总DNA电转化大肠杆菌,筛选共获得38个BAC分子克隆化病毒,提取BAC-DNA转染鸡胚成纤维细胞以拯救重组病毒。
The construction of DNA vector systems such asλ bacteriophage,cosmid,P1-derived artificial chromosome,yeast artificial chromosome , bacterial artificial chromosome(BAC)system has been developed as another powerful DNA vector system.
细菌人工染色体是继 λ噬菌体、黏粒、噬菌体人工染色体、酵母人工染色体等载体系统之后发展起来的 DNA载体系统 ,以其容量大、遗传特性稳定、嵌合体少、插入片段易回收、操作简便等优点 ,而被广泛应用于基因组较大的真核生物基因组研究中 ,并发挥着前所未有的重要作用。
Objective: To set up a kind of improved method of construction of bacterial artificial chromosome(BAC) library.
目的:建立一种改进的更简便、易操作的细菌人工染色体(BAC)文库构建方法。
Construction of Artificial Chromosomes of Euplotes Octocarinatus Containing GFP Gene;
八肋游仆虫绿色荧光蛋白（GFP）人工染色体的构建
Research on the Preparation of Vector DNA about Transformation-competent Artificial Chromosome (TAC) Library;
可转化人工染色体（TAC）文库载体DNA制备的研究
Construction of the Genomic Bacterial Artificial Chromosome Library of Maize Huang Zao Si;
玉米黄早四基因组细菌人工染色体文库的构建
Cloning of Marek s Disease Virus Genome as an Infectious Bacterial Artificial Chromosome;
鸡马立克氏病病毒细菌人工染色体的构建
Construction of a Bacterial Artificial Chromosome Library of Cotton Cultivars;
棉花品种细菌人工染色体（BAC）文库的构建
Construction and Screening of a Transformation-Competent Artificial Chromosome (TAC) Library of Zizania Latifolia (Griseb.);
菰基因组可转化人工染色体文库的构建及筛选
Construction and Idetification of Chinese Game Chicken Bacterial Artificial Chromosome Library
中国斗鸡细菌人工染色体文库构建及鉴定
Optimizing the Constructing Condition of Bacterial Artificial Chromosome Library in the Cashmere Goat
绒山羊细菌人工染色体BAC文库构建条件的优化
Construction and Analysis of Bacterial Artificial Chromosome(BAC) Library of Nosema bombycis
家蚕微孢子虫细菌人工染色体文库的构建
Construction of a bacterial artificial chromosome (BAC) genomic library of Heilongjiang River carps (C. carpio haematopterus)
黑龙江野鲤细菌人工染色体基因组文库构建
Construction of an Euplotes octocarinatus Macronuclear Artificial Chromosome Harboring Codon-optimized EGFP Gene
八肋游仆虫含绿色荧光蛋白基因的大核人工染色体的构建
Progress on Application of Bacterial Artificial Chromosome System in the Reverse Genetics
细菌人工染色体载体系统在反向遗传学中的应用研究进展
Study on chromosome damage in workers exposed to vinyl chloride monomer;
氯乙烯暴露对作业工人染色体的损伤
Chromosome aberration analysis of peripheral lymphocytes in workers exposed to benzene
苯接触工人外周血淋巴细胞染色体畸变分析
Genetic Susceptibility of Chromosome Damage in Vinyl Chloride Monomer Exposed Workers;
氯乙烯接触工人染色体损伤的遗传易感性研究
Chromosome Analysis Based on Fuzzy Theory and Neural Artificial Network;
基于模糊理论和人工神经网络的染色体分析
Modification of Genetic Polymorphisms on Chromosomal Damage in Vinyl Chloride Monomer Exposed Workers;
基因多态对氯乙烯接触工人染色体损伤的修饰
Measurement of content of uranium in urine of workers in an uranium mine and analysis of aberration rate of their chromosomes;
铀矿工人尿铀含量测定与染色体畸变率分析