Cultivated three kinds of lactic acid bacteria(LAB) in order to searching for the multiplication factors.
确定了提取姬松茸多糖的最佳工艺参数,即温度100℃,时间5h,pH值为10;研究了多糖对乳酸菌生长的影响,采用DEAE-纤维素柱层析分离姬松茸粗多糖,得到3种多糖,通过对乳酸菌的培养寻找增殖因子;测定乳酸菌的生长曲线、菌液OD值与pH值,比较了3种多糖对乳酸菌的促生长作用的差异。
It cultivated two kinds of lactic acid bacteria(LAB) with a kind of special medium which was added with 1 percent of PS as multiplication factor,cultivated at 37℃ and compared with blank sample.
实验采用DEAE-纤维素柱层析法分离食用菌提取物,得到3种多糖,通过对在培养乳酸菌的MRS培养基中添加1%3种多糖作为增殖因子,并作空白对照,37℃恒温培养,测定乳酸菌的生长曲线、菌液OD值与pH值,比较3种多糖对乳酸菌的促生长作用的差异得出,多糖Ⅰ、多糖Ⅱ对乳酸菌的增殖作用比较明显,多糖Ⅲ的增殖作用因大分子多糖与蛋白-多糖复合物含量较大而次之,但是3种多糖培养乳酸菌对数期期间活菌数都达到了1010cfu/mL以上,培养终止时间为12h。
Cultivation and Proliferation-promoting Cytokines of HPP-EPC from Murine Bone Marrow
小鼠骨髓高增殖潜能内皮祖细胞的培养与促增殖因子研究
Nuclear Factor-κB in Proliferation and Apoptosis in Rat Hepatic Stellate Cells;
核因子κB与肝星状细胞增殖及凋亡
Preparation and Rapid Vegetative Propagation of Transgenic Laminaria Japonica Gametophytes
转基因海带配子体的制备与高效增殖
NSPc1 is an essential factor for HeLa cells' proliferation
NSPc1是维持HeLa细胞正常增殖的必要因子
Primary Study on Inhibitory Effect of Platelet Factor 4 on U937 Cells Proliferation;
血小板因子-4抑制U937细胞增殖的机理研究
Key Features Extraction from Algae Proliferation Process and Its Application;
藻类增殖过程中关键因子的提取与应用研究
Mechanism of Proliferation and Metastasis Effect Caused by LRP16 Genein Breast Cancer;
LRP16基因促进乳腺癌增殖、转移分子机制的研究
Study on the Proliferation of CFU-GM and Related Regulatory Cytokines in AML Patients;
AML患者CFU-GM增殖与相关调控因子的研究
Role of Nuclear Factor Kappa B in the Proliferation of Neural Stem Cells;
核因子κB在神经干细胞增殖过程中的调控作用
Effects of Different Factors on HeLa Cell Proliferation, Apoptosis and HSP70 Expression;
不同因子对HeLa细胞增殖、凋亡和HSP70表达的影响
Influence of Midkine on Proliferation and Apotosis of Lung Adenocarcinoma Cell Lines A_(549)
中期因子对肺腺癌A549细胞增殖凋亡的影响
Effect of different influential factors on induction and multiplication of Paeonia suffruticosa buds in vitro
牡丹鳞芽诱导与增殖过程中影响因子研究
Redox factor 1 increases proliferation of neonatal rat cardiac fibroblasts
氧化还原因子1促进乳鼠心肌成纤维细胞增殖
Factors Influencing the Proliferation of Cymbidium goeringii Protocorm in Vitro
离体培养春兰原球茎增殖的影响因子研究
Proliferative effects of growth factors on muscle-derived stem cells in vitro
生长因子对骨骼肌肌源性干细胞增殖的影响
EFFECTS OF HUMAN HSF1 ON TRANSCRIPTIONAL REGULATION OF PROHIBITIN PROMOTER
人热休克因子1对抗增殖蛋白基因启动子转录调控的研究
A Study of Modulation of Augmenter of Liver Regeneration on Cell Proliferation and Its Relationship between Na～+, K～+-ATPase;
肝再生增强因子调控细胞增殖及其与钠、钾ATP酶关系研究
Effect of Human Augmenter of Liver Regeneration on Proliferation of Human PBMCs and Its Mechanism
人肝再生增强因子对人外周血单个核细胞增殖的影响及其机制