Based on the principle that the rotation of the single enantiomer was proportional to its concentration under the certain circumstance,combined high performance liquid chromatography(HPLC) with online polarimetric detector,a new(procedure) was developed for real-time e.
根据在一定测定条件下,单一对映体旋光度仅与其浓度成正比,利用高效液相色谱与旋光检测器联用,提出了一种实时测定e。