To obtain the recombinant vulpes vulpes growth hormone.
为了获得重组的银狐生长激素,本试验用Trizol的方法从银狐脑垂体组织中提取总RNA,以mRNA为模板,用RT-PCR方法扩增出生长激素成熟cDNA片段并克隆到载体质粒pMD18-T中;通过含有BamHⅠ酶切位点的特异引物从pMD18-T-fGH中亚克隆出cDNA片段,并将其重组到pPROEXTMHta质粒中,构建了银狐生长激素原核表达质粒pPRO-EXTMHta-fGH。
Growth hormone gene of Vulpes vulpes was sub-cloned by polymerase chain reaction from recombined pMD18-T-GHcDNA plasmid, and then inserted into eukaryotic expressed vector pcDNA3.
用PCR方法,从含有银狐生长激素mRNA的阳性重组质粒pMD18-T-fGH中亚克隆出生长激素基因,然后定向插入到真核表达载体pcDNA3。
A pair of primers were designed to amplify the sequences of melatonin receptor 1A from arctic fox and silver fox according to the sequence homology of MTNR1A from other animal species.
根据其它物种褪黑激素受体(melatonin receptor,MTNR)1A基因核酸序列的同源性设计引物,PCR扩增蓝狐和银狐MTNR1A基因的546 bp DNA序列,GenBank登录号分别为EU170442和EU170443。
Average diameter of cell nucleus of the pineal body with Silver fox is 5.
本研究初步观察了犬科银狐和貉松果体的形态位置和组织结构,通过比较认为,两者的松果体形态位置相近,2种动物松果体的差异之处是:银狐松果体体积略小,细胞密度最小,毛细血管最丰富,银狐松果体细胞核直径平均为5。
By selecting appropriate extermal hormone and mate, inject female Silver fox use proper dosage on right time to make Silver fox ovulate more and get more whelps, thereby improve the propagation ability of Silver fox.
通过选择合适的外源激素及配比,并适时对银狐母体注射,可使其超数排卵,提高产仔数量,从而提高银狐繁殖力。