The stable isotope labeling technique provided an ideal method for accurately quantitating the low abundance proteins which play a key role in cells or tissues.
近几年来,定量蛋白质组的技术发展很快,稳定同位素标记技术的提出,为准确定量在细胞或组织体系中发挥重要功能的低丰度蛋白质提供了一个理想的方法。
Methods Based on the fact that 86Rb has strong affinity to myocardium, isotope labeling method was applied to study the effect of MELAI on nutritional bloodflow of myocardium in mice.
②方法根据86Rb对心肌亲和力强的特点,采用同位素标记方法研究贻贝多活素2,4g/kg体重对小鼠心肌营养性血流量影响。
At present,stable isotope labeling is probably considered as one of the most accurate ways to relatively quantify protein expression levels and additionally stable isotope labeling may be directly combined to LC MS/MS approaches.
目前,应用稳定同位素标记技术定量蛋白质表达水平是最准确的方法之一,且联合色谱质谱技术更具优势。
The technique based on stable isotope labeling and mass spectrometry is one of the main supporting techniques for protein quantification.
基于稳定同位素标记的质谱分析技术是蛋白质定量最常用的方法之一。
isotopically tagged
同位素示踪的, 同位素标记的
Telomere length measurement of human leukocyte in peripheral blood by non-radio -isotopic hybridization;
非同位素标记探针杂交分析白细胞端粒DNA长度
Evaluation on False-Positive of Isotope-Labeled Peptides Using Least-Square Method
利用最小二乘法判断同位素标记肽的假阳性
Isotopically Labeled Synthesis and Identification of Two Novel Pesticides;
新农药丙酯草醚和苦皮藤素同位素标记物的合成与鉴定
Determination of Lipoprotein Lipase Activity Using Radioactive Substrate Emulsion and Its Application
放射性同位素标记法检测脂蛋白脂肪酶活性及其应用
Study of Preparation of Segmental Isotopic Labeling ApoE with Intein-mediated Protein Ligation and Structure of RAP;
蛋白质拼接法制备片段同位素标记ApoE及RAP结构的初步研究
Application and Development of the SILAC Technique in Proteomics
细胞培养稳定同位素标记技术在蛋白质组学中的应用与进展
Development of Protein Quantitative Algorithms Based on Stable Isotope Labeling and Mass Spectrometric Analysis
基于稳定同位素标记与质谱分析的蛋白质定量算法研究进展
Stable Isotope Iodine-labeled Antibodies-ICP-MS for Immunoassay;
稳定同位素碘标记-ICP-MS在免疫分析中的应用
Treatment Methods of Isotope ~(15)N Labeled Sample for Mass Spectrometry
质谱检测用同位素~(15)N标记样品的处理方法
Apoplast Calmodulin Localization in Transgenic Tobacco Cells Using GFP as Reporter;
利用GFP标记对胞外钙调素定位的研究
Historical eutrophication in Wuli Bay of Taihu Lake, China based on carbon isoto pe record.
太湖五里湖富营养化的碳同位素记录
Carbon and Oxygen Isotope Variations of Devonian Seawater:Isotopic Records from Brachiopod Shell
泥盆纪海水的碳、氧同位素变化——来自腕足化石的同位素记录
Location of Pigment Gene for Wheat with Purple Grains Using Microsatellite Marker;
利用微卫星标记定位紫粒小麦的色素基因
Subpixel location algorithm for circle target center based on spatial moment
基于空间矩的圆标记中心亚像素定位算法
The in situ hybridization effort estimation between antisense RNA probe and random primed DNA probe;
不同方式标记非放射性探针原位杂交效果评价
Effects of Interval Mapping for Quantitative Trait Loci under Different Heritabilities and Marker Densities
不同遗传力和标记密度下QTL区间定位的效果
A certificating study on La-Ce rock reference material and Ce isotopic standa rd solution
La-Ce法岩石标准物质和Ce同位素标准溶液研制