The ubiquitin gene of Helicoverpa armigera single-nucleocapsid nucleopolyhedrovirus(HaSNPV) was amplified by PCR.
通过PCR方法,克隆了棉铃虫核多角体病毒(HaSNPV)泛素基因(Ubiquitin,Ubi)。
Digestion of Helicovrtpa armigera nuclear polyhedrosis virus(HaSNPV Hubei isolate)with 11 restrictionendonucleases,BamH Ⅰ,BstE Ⅱ,EcoR Ⅰ,ECoR Ⅴ,Hind Ⅲ,Kpn Ⅰ,Pst Ⅰ,SalⅠ,SstⅠ,Xba Ⅰ andXho Ⅰ resulted in 10, 12, 22,21, 13, 6, 6, 40, 6, 21 and 6 fragments,respectively.
应用１１种限制性内酶ＢａｍＨⅠ、ＢｓｔＥⅡ、ＥｃｏＲⅠ、ＥｃｏＲⅤ、ＨｉｎｄⅢ、ＫｐｎⅠ、ＰｓｔⅠ、ＳａｌⅠ、ＳｓｔⅠ、ＸｂａⅠａⅠ和ＸⅠⅠ和ＸｈｏⅠ分别将中国棉铃虫核多角体病毒（单粒包埋ＨａＳＮＰＶ）湖北株基因组Ｄ组ＤＮＡ酶切为１０、１２、２２、２１、１３、６、６、４０、６、２１、６个片段，并求得基因组大小平均为１什什Ｍｒ≈７９。
The 741 bp coding region of polyhedrin gene (ph) was amplified from Euproctis pseudoconspersa nucleopolyhedrovirus(EupsNPV)genome and its prokaryotic expression vector pET-28-a-Ep-ph was constructed.
以茶毛虫核型多角体病毒(EupsNPV)的基因组DNA为模板,PCR扩增出全长为741bp的ph基因编码区片段。
Identification of the Vertical Transmission of HaNPVin Helicoverpa armigera by PCR Assay;
PCR法证实棉铃虫核型多角体病毒对棉铃虫经卵和蛹的垂直传播
otal RNAs were isolated from Heliothis armigera larvae infected with Heliothis armigeraNuclear Polyhedrosis Virus(HaNPV) late after infection(60 h postinfection),800 μg total RNAswere obtained from 1g larvae by improved granidinium isothiocgarate method.
棉铃虫核型多角体病毒ｍＲＮＡ的快速制备与体外翻译吴金炉，刘润忠，张友清（中国科学院武汉病毒研究所，武汉４３００７１）关键词棉铃虫核型多角体病毒，ｍＲＮＡ，快速制备，体外翻译，ＥＬＩＳＡ在昆虫杆状病毒ｍＲＮＡ的结构与功能研究方面，主要是以ＡｃＮＰＶ为研。
Expression of Helicovapa Armigera Nucleopolyhedravirus (HearNPV) HA2 in Insect Cell;
棉铃虫核多角体病毒HA2蛋白表达分析
Structural and Functional Analysis of ORF44(Ha44)of Helicoverpa Armigera Nucleopolyherovirus (HearNPV);
棉铃虫核多角体病毒ORF44（Ha44）的结构与功能研究
The Analysis of HaSNPV ODV Infection Hz-AM1 Cells in Vitro;
棉铃虫核多角体病毒包埋型病毒粒子体外感染Hz-AM1细胞的研究
Transmission Dynamics of Helicoverpa Armigera Nucleopolyhedrovirus and Construction of a Recombinant Virus Expressing Superoxide Dismutase;
棉铃虫核多角体病毒的传播动态及含SOD重组病毒的构建
Cloning and Expression of Ubiquitin Gene of Helicoverpa Armigera Single-nucleocapsid Nucleopolyhedrovirus and Its Antiserum Preparation;
棉铃虫核多角体病毒泛素基因的克隆表达及抗体制备
Studies on the Safety of Recombinant Helicoverpa Armigera Single Nucleocapsid Nucleoplyedrovirus (HaSNPV) Released into Environment;
重组棉铃虫核多角体病毒环境释放的安全性研究
Function Analysis of Two Unknown Genes of HaSNPV, Ha94 and Ha132;
棉铃虫核多角体病毒的两个保守基因Ha94与Ha132的功能分析
Mechanism of Helicoverpa Armigera Nucleopolyhedrovirus Triggered Se-UCR Apoptosis Blocked by Spodoptera Exigua Nucleopolyhedrovirus;
甜菜夜蛾核多角体病毒抑制棉铃虫核多角体病毒诱导的细胞凋亡及其分子机理研究
Analysis of bro Genes from Helicoverpa Armigera Single Nucleocapsid Nucleopolyhedrovirus (HaSNPV);
棉铃虫单核衣壳核多角体病毒bro基因的研究
Characterization of ORF128 and ORF135 from Helicoverpa Armigera Single Nucleocapsid Nucleopolyhedrovirus (HaSNPV);
棉铃虫核型多角体病毒ORF128、ORF135等基因的特性分析
Purification and Identification of a Nucleopolyhedrovirus Isolated form Helicoverpa armigera
一种棉铃虫多角体病毒的纯化与鉴定
Sequence Analysis and Bacterial Expression of ORF34 and ORF44 from Helicoverpa Armigera Nucleopolyhedrovirus;
棉铃虫核型多角体病毒ORF34、ORF44的序列分析与原核表达
On the Function of the ORF44 Gene of Helicoverpa Armigera Nucleopolyhedrovirus: A Putative Functional Gene;
棉铃虫单核衣壳核型多角体病毒（HaSNPV）orf44基因的研究
Preliminary Study on the Function of Ubquitin Encoded by Heliothis Armingera Single-capsid Nuclear Polyhedrosis Virus;
棉铃虫单核衣壳核型多角体病毒编码的泛素功能初探
The Construction of Heliothis Armingera Single-capsid Nuclear Polyhedrosis Recombinant Virus Lacking Cathepsin;
缺失v-cath基因的棉铃虫单核衣壳核型多角体重组病毒的构建
Cloning and Expression of ORF115a and ORF127 Gene from Helicoverpa Armigera Nucleocapsid Nucleopolyhedrorius;
棉铃虫核型多角体病毒ORF115a和ORF127基因的克隆及表达
The Study of Association of the Replication of HaSNPV with Nuclear Matrix in Hz Cells;
中国棉铃虫单粒包埋核型多角体病毒的复制与宿主细胞核骨架相互关系的研究
Cloning,Sequence Analysis and Protein Tertiary Structure Prediction of 38k Gene from Helicoverpa armigera Multipie Nuclear Polyhedrosis Virus
棉铃虫多核型多角体病毒38k基因的克隆、序列分析及其蛋白高级结构的预测