Methods hESC were seeded on mouse embryonic fibroblasts (MEF),human marrow stromal cells (hMSC),and human foreskin fibroblasts (hFF),respectively.
方法分别用小鼠胚胎成纤维细胞(MEF)、人包皮成纤维细胞(hFF)、人骨髓间充质细胞(hMSC)作为滋养层培养hESC系H9细胞,采用碱性磷酸酶染色和台盼蓝染色观察不同滋养层支持的hESC的碱性磷酸酶阳性克隆数、细胞产量和细胞存活率,并采用免疫荧光染色对传代5代以后的hESC进行生物学鉴定。
Rat dermal fibrobalasts and human neofetus dermal fibroblasts were cultured in vitro to evaluate the biocompatibility of the chitosan films.
为考察壳聚糖对皮肤成纤维细胞的相容性 ,采用流延法制备了纯壳聚糖膜和含甘油的壳聚糖膜 ,并在所制备的壳聚糖膜上进行了大鼠皮肤成纤维细胞和人胎儿皮肤成纤维细胞的体外培养 。
In vitro Culture of Toxoplasma gondii Tachyzoites in HFF and Hela Cells
人包皮成纤维细胞和人子宫颈癌细胞体外培养弓形虫速殖子
Cell Cycle Analysis of in Vitro Cultured Human Adult Skin Fibroblasts
体外培养成人包皮成纤维细胞周期同步化效果检测
The Study of Human Foreskin Fibroblasts Biologic Character and Effect of Human Foreskin Fibroblast as Feeder Layer for Human Embryonic Germ Cells
包皮成纤维细胞生物学特性研究及以其为饲养层对胚胎生殖细胞生长的影响
Cloned Sheeps from Adult Skin Fibroblast Cells;
成年皮肤成纤维细胞克隆绵羊的研究
The Production of Cloned Sheep from Adult Skin Fibroblast Cells
成年皮肤成纤维细胞克隆绵羊的生产
The Expression and Research of Dystrophin Gene in Dermal Fibroblast Cells
皮肤成纤维细胞中dystrophin基因的表达研究
Isolation,culture and identification of human fibroblasts
人真皮成纤维细胞的分离培养及鉴定
Study on Migration of Skin Fibroblasts with Telomerase Reconstitution
端粒酶重建皮肤成纤维细胞迁移研究
Culture and cryopreservation of rabbit conjunctival epithelial cells and fibroblasts
兔眼结膜上皮细胞和成纤维细胞的培养及冻存
Study on Effect of Percutaneous Implant on Rat Skin Fibroblasts
经皮植入体对大鼠皮肤成纤维细胞的作用研究
Studies Related to Stem Cells Derived from Somatic Cells Nuclear Transfer Embryos and Adult Fibroblasts
体细胞核移植胚胎和皮肤成纤维细胞来源干细胞的相关技术研究
Culture of human embryonic cells by using human dermal fibroblasts as feeder cells
以人皮肤成纤维细胞作为饲养层细胞培养人胚胎干细胞
Effects of AcSDKP on proliferation and synthesis of collagen in human dermal fibroblasts
AcSDKP对人真皮成纤维细胞增殖及胶原合成的影响
Experimental Study on the Transplantation of a Composite Skin Graft Composed of Cultured Human Keratinocytes and Fibroblasts and a Chitosan-Collagen Matrix;
表皮细胞、成纤维细胞和几丁质—胶原蛋白复合人工皮移植实验研究
Effects of Intense Pulse Light on the Human Fibroblasts and Vascular Endothelial Cells;
强脉冲光对皮肤成纤维细胞及血管内皮细胞的作用
Effect of paeonol on the expression of MMP-9 mRNA and cytokines production in human skin fibroblasts induced by TNF-α
丹皮酚对TNF-α诱导真皮成纤维细胞MMP-9mRNA及细胞因子表达的影响
Studies on the Growth of Rabbit Skin Fibroblasts on the Surfaces of Acellular Dermal Matrix
兔皮肤成纤维细胞在脱细胞真皮支架材料表面生长的研究
Culture and Gene Transfection of Adult Epidermal Keratincytes and Dermal Fibroblasts in Vitro;
原代成体皮肤角质形成细胞、成纤维细胞的培养和转基因研究