Objective:To establish Allele-specific amplification(ASA-PCR)for study the distribution of MDR1 polymorphism in transplant patients.
目的:建立等位基因特异扩增法(Allele-Specific Amplification,ASA-PCR)研究移植患者多药耐药基因(multi-drug re- sistance gene,MDR1)多态性。
[Methods] A total of 100 healthy Liaoning Hans were studied by the use of PCR and allele specific amplification (ASA) techniques.
方法用聚合酶链反应及等位基因特异性扩增技术对100名辽宁地区汉族人的DRD4基因启动子区3个多态性进行了检测,并与其他人群做了比较。
Allele specific amplification (ASA) is a low-cost, robust technique that can be utilized to discriminate between the all.
本试验通过等位基因特异扩增(ASA)方法对9个香稻和3个非香稻品种(品系),以及3个香稻/非香稻组合的F1的香味基因,进行快速检测。
Multiplex SNP typing using monotube allele-specific amplification
单管等位基因特异性扩增法同时测定多重单核苷酸多态性
Microchip electrophoresis coupled with multiplex allele-specific amplification for typing multiple single nucleotide polymorphisms(SNPs) simultaneously
多重等位基因特异性扩增——微流控芯片电泳法同时测定多个单核苷酸多态性位点
An accurate and effective method was developed for rapidly genotyping in a single tube, which was based on di-allele-specific amplification with artificially modified primers.
该方法在等位基因特异性扩增法的基础上,在内引物3′端引入了一个人工错配 碱基并在5′端附加一段“尾巴”作为引物的锚定部分,使扩增反应的特异性得到了提高。
ectopic amplificatio
异位扩增[引物与非目标序列结合而引导的PCR扩增]
Optimizing the conditions for in vitro expansion of HBV protein epitope-specific CTLs
乙肝病毒蛋白抗原表位特异性CTL体外扩增条件优化
Multiplex SNP typing using monotube allele-specific amplification
单管等位基因特异性扩增法同时测定多重单核苷酸多态性
Microchip electrophoresis coupled with multiplex allele-specific amplification for typing multiple single nucleotide polymorphisms(SNPs) simultaneously
多重等位基因特异性扩增——微流控芯片电泳法同时测定多个单核苷酸多态性位点
Study on polymorphism of human eighth complement component(C8A)by using allele specific amplification method;
用等位基因特异扩增技术检测人类补体第八成份C8A DNA多态性的研究
Adapter-ligation Mediated Allele-specific Amplification (ALM-ASA) for Multiplex SNP Genotyping of LRRK2
应用基于适配器连接介导的等位基因特异性扩增法检测LRRK2基因的单核苷酸多态性
Analysis of a variation case when using the Profiler Plus kit to amplify the Amelogenin loci;
Profiler Plus试剂盒扩增Amelogenin基因座变异1例
Amplification of flanking sequence of the transposon Tn917 insertion sites
转座子Tn917插入位点侧翼序列的扩增
Amplification of Pseudo attP Site Using Adaptor PCR
应用接头PCR的方法扩增假attP位点
A Commotion Occurred in Amplification of Consecutive Polymerase Chain Reaction (PCR): The Mechanism and Implications;
连续PCR扩增过程中异常现象的发生机理研究
ANALYSIS ON THE NEW ECONOMIC GROWTH THEORY OF REGIONAL DISPARITY IN CHINA;
我国地区差异扩大的新经济增长理论分析
Fingerprint image enhancement method based on anisotropic tensor reverse diffusion
各向异性张量逆扩散指纹图像增强方法
Induction,proliferation and functional study of specific anti-acute myeloid leukemia(AML) T cells
抗AML特异性T细胞的诱导、扩增及功能研究
Preliminary Research on Gold Nanoparticles Enhancing the Specificity of PCR Amplification of Low Copy Gene from the Complicated System
纳米金增强复杂体系中PCR扩增低拷贝基因的反应特异性初步研究
continuing to encourage residents to increase consumption and expanding the channels of employment;
继续鼓励居民扩大消费，多渠道增加就业岗位；
Evaluation of 9 STR Loci in paternity identification;
复合扩增9个STR位点在亲子鉴定中的应用评估
Amplified Fragment Length Polymorphism Analysis of ApoB Locus in Tissue and Its Application in Forensic Science;
组织ApoB位点扩增片段长度多态性分析及其应用
Isolation of Microsatellite Loci from Pratt's Leaf-nosed Bat (Hipposideros Pratti) and Cross-species Amplification
普氏蹄蝠（Hipposideros pratti）微卫星位点的筛选及交叉种扩增