The PCR primers design and identification for the detection of beer spoilage lactic acid bacteria;
啤酒有害乳酸菌PCR引物的设计与验证
Amplification efficiency test for primers in real-time PCR reactions;
鉴定定量PCR不同引物对的扩增效率
Screening of Random Primer and Optimizing of Reaction System for Arbitrarily Primed Polymerase Chain Reaction on 8 Sorts of Bacteria;
细菌随机引物聚合酶链反应分析中随机引物的筛选与反应体系的优化
3 -terminus shifted bases degeneracy primers increasing sensitivity of polymerase chain reaction;
3’末端碱基游移混合引物提高多变区基因片段PCR检测的阳性率(英文)
Optimization for SRAP-PCR system in Cynodon dactylon and selection of primers;
狗牙根SRAP-PCR反应体系优化及引物筛选
Selection of primers and establishment of SSR-PCR reaction system on Pinus tabulaeformis Carr.;
油松SSR-PCR引物筛选及反应体系的建立
Detecting enterovirus by reverse transcription nested-polymerase chain reaction established with universal primers;
应用通用引物建立巢式逆转录聚合酶链反应检测肠道病毒(英文)
A rapid quantitative polymerase chain reaction(QPCR) analysis method with universal primers was developed to detect cell densities of the enteric pathogenic bacteria from 5 surface water of Xi'an City for 4 months continuously.
采用通用引物,通过实时荧光定量PCR方法(QPCR),对西安市5处地表水体中肠道病原细菌的细胞密度进行4个月的连续检测,并将QPCR检测结果与滤膜法测得的大肠菌群CFU值进行比较分析。
In order to investigate the application values of polymerase chain reaction(PCR) technique in detection of pathogenic bacteria in surface water,the universal primers were designed and synthesized according to the high conversation of 16S rRNA gene.
为了探索通用引物PCR方法在地表水病原细菌检测中的应用价值,利用16S rRNA基因的高度保守性,设计并合成细菌的通用引物,采用合成的引物扩增参考菌株及西安市区不同地表水样,并对PCR产物分别进行序列测定及序列同源性分析,同时检测水样中的细菌总数和粪大肠杆菌浓度。
The study is to assess the effects of the important primer parameters such as GC contents,Tm and secondary structure,which were showed by primer designe software Primer 5.
利用引物设计软件Primer 5。
Due to the lack of polyadenylation in prokaryotic mRNA, the primer design of prokaryotic DDRT-PCR is different from eukaryocyte.
由于原核细胞mRNA3'端不存在ploy(A)结构,因而原核细胞DDRT-PCR引物设计不同于真核细胞。
A primer design method was developed for detection of point mutation in rice.
介绍了一种用于水稻点突变检测的PCR引物设计方法。
Based on the sequence of internal transcribed spacer regions(ITS)of the ribosomal gene,a couple of specific primers for P sojae was synthesized,and a single band of PCR product about 330 bp was amplified from the crashed sample of zoospore or oospores carried in the soil or the diseased soybean tissues.
利用该特异引物可稳定地从含有大豆疫霉游动孢子或卵孢子的土壤及其发病组织中检测出病原菌。