Determination of concentration of ATP by immobilized firefly luciferase reactor;
固定化虫荧光素酶反应器测定 ATP 的初步研究
To assess quickly the potential environmental hazard of heavy metal pollutant by the gene recombinant cell, a reporter vector regulated by electrophile response elements ( EPRE) was constructed by fusing EPRE to the TK minimal promoter and the gene encoding firefly luciferase (LUC) with biomolecular techniques.
研究用基因重组细胞快速评价重金属污染物对健康的危害,采用分子生物学方法,将亲电性化学反应元件(EPRE)与TK基本启动子和编码虫荧光素酶(LUC)基因相融合,构建成EPRE调控的LUC报告载体,将其转染于HeLa细胞。
Study on the Trehalose Protection on Activity of Luciferase;
海藻糖对虫荧光素酶活性保护作用研究
Objective To study the DTA vector containing the Ewing family of tumor s EWS-FLI1 binding sequence depresses the co-transferred luciferase reporter plasmid.
结果 每增加一个 pS2 DTA剂量梯度 ,在尤文肉瘤细胞中都可以检测到虫荧光素酶的表达显著降低 ,而在对照细胞中这种作用却不明显 ;在同一个pS2 DTA剂量梯度下 ,虫荧光素酶在尤文肉瘤细胞的表达都显著高于非尤文肉瘤细胞。
Methods The transfection of luciferase reporter plasmid(pS2),which contains the EWS FLI1 binding sequence,into cultured Ewing′s sarcoma cell lines(RD ES,SK ES) and control cell lines (Hela,NIH3T3)was performed by liposome method and luciferase activity in the cellular lysate was measured by spectrofluorometer.
结果 虫荧光素酶在尤文肉瘤细胞中表达强度明显高于非尤文肉瘤细胞。
The article researches the affect deletion of N terminal amino acids of firefly luciferase on activity of the enzyme.
由于Ｎ末端缺失６个氨基酸的突变体保持了７７％的酶活性，因而萤火虫荧光素酶Ｎ末端第７个氨基酸与酶的催化活性密切相关。
Objective: To study the agreement between chemiluminescence assay(Che) and bioluminescence imaging(Bio) used for detecting the firefly luciferase(Fluc) activity.
目的:比较2种萤火虫荧光素酶活性检测方法的一致性。
In this research,the luc gene coding firefly luciferase(LUC) was amplified by using the DNA of Luciferase reporter vector pXP2 as the template.
以荧光虫荧光素酶报告载体pXP2 DNA为模板,扩增得到编码萤火虫荧光素酶(LUC)的基因(luc),构建了诱导型表达载体pQE30-luc。
A Study on the Properties and Applications of Firefly Luciferase;
萤火虫荧光素酶的性质和应用的研究
Construction of Recombinant Retroviral Vector Containing Firefly Luciferase Reporter Gene
萤火虫荧光素酶报告基因重组逆转录病毒载体的构建及鉴定
An enzyme present in the cells of bioluminescent organisms that catalyzes the oxidation of luciferin.
荧光素酶存在于生物性发光组织的细胞中，催化荧光素氧化的酶
Transcriptional activation activity of variant was evaluated through methods of relative activity of luciferase.
相对荧光素酶法检测转录激活活性。
Construction of Dual-Luciferase reporter plasmid assay system for human NOX1 gene
NOX1基因荧光素酶报告基因系统的建立
Studies on Fluorescence Investigation of the Interaction of Artemisinin with Peroxidase
青蒿素与过氧化物酶作用的荧光研究
Fluorogenic quantitative PCR method for Plasmodium vivax
间日疟原虫荧光定量聚合酶链反应检测方法的建立
Construction and identification of luciferase reporter gene vector containing HMGB1 promoter
HMGB1启动子荧光素酶报告基因的构建及鉴定
Optimization of the expression of luc gene in E. coli
荧光素酶基因luc在大肠杆菌中表达条件优化
Viviperception of Transgenic Human Embryonic Stem Cells Labeled by Luciferase
荧光素酶标记转基因人胚胎干细胞的活体观察
Creation and Identification of Luciferase Reporter Gene Containing Human Bax Promotor
人Bax启动子荧光素酶报告基因的构建和鉴定
Construction and identification of human Puma promotor luciferase report gene vector
人Puma启动子荧光素酶报告基因的构建和鉴定
Primary Study on Rapid Bioluminescence Evaluation of Disinfection Effect Using Luciferase;
荧光素酶生物发光法快速评价消毒效果的初步研究
Quantitative detection of NADH by in vitro bacterial luciferase bioluminescent
细菌荧光素酶体外发光体系的建立及应用于NADH定量检测
The Effect of Optical Brightener, Host Plants and Geographic Strains of LdNPV on the Phenoloxidase and Midgut Esterase Activity of Gypsy Moth Larvae
荧光增白剂、寄主植物、LdNPV地理品系对舞毒蛾幼虫酚氧化酶和中肠酯酶的影响
Construction of luciferase reporter gene plasmid containing hBD-2 and mutant correlated with Crohn's disease
β-防御素-2荧光素酶报告基因载体及克罗恩突变体的构建
The transcriptional activation activity of GR was evaluated through method of relative activity of luciferase.
相对荧光素酶活性法检测GR转录激活功能的变化。
The transcriptional activation function of GR was evaluated through the detection of relative luciferase activity after dexamethasone treatment.
相对荧光素酶活性法检测地塞米松作用后GR转录激活功能的变化。