In order to obtain the whole genome of Antheraea pernyi nucleopolyhedrovirus (ApNPV), we established the genomic library by digesting the genome DNA with BamHⅠ and SalⅠ and cloning with pGEM-3Z.
为获得柞蚕核型多角体病毒(Antheraea pernyinucleopolyhedrovirus,ApNPV)基因组序列,采用随机克隆方法,建立ApNPV的质粒基因文库,并通过对插入片段进行克隆鉴定和序列分析,获得了编码包涵体衍生型病毒特异囊膜蛋白基因odv-e56。
The gene encoding single-stranded DNA-binding protein(SSB) late expression factor 3(lef-3) was cloned from PstⅠ and XhoⅠ fragment of Antheraea pernyi nucleopolyhedrovirus(ApNPV) genome.
用随机克隆法,克隆得到了柞蚕核型多角体病毒(Antheraeapernyinucleopolyhedrovirus,ApNPV)PstⅠ和XhoⅠ片段,经序列分析表明,该片段含有完整的晚期表达因子3(lef3),与其它来源的lef3基因具有很高的同源性。
7 kb fragment of IE promoter(IE1)was got from ApNPV by PCR and subcloned into pMD18-T and sequenced.
以柞蚕核型多角体病毒(ApNPV)DNA为模板,运用PCR技术钓取ApNPV(IE-1)基因的启动子片段,并将其克隆到pMD18-T载体上进行测序分析。
In order to identify the difference on the infection and genes between Antheraea pernyi nucleopolyhedrovirus(ApNPV) and Philosamia cynthia ricini NPV(PcrNPV),we carried out these experiments.
为了解柞蚕核型多角体病毒(Antheraea pernyinucleopolyhedrovirus,ApNPV)和蓖麻蚕核型多角体病毒(Philosamia cynthia riciniNPV,PcrNPV)在感染性及基因水平上的差别,克隆了PcrNPV解旋酶基因helicase,对该基因进行了测序及同源性分析。
With the method of random cloning of the genome of Antheraea pernyi nucleopolyhedrovirus(ApNPV) sequence,we got a fragment including pe-38,ie-2 and the promoter of pe-38 by measuring and megaligning the inserted pGEM-3Z vector sequence.
采用随机克隆方法,通过对插入pGEM-3Z载体的柞蚕核型多角体病毒基因组的部分片段进行测序和序列分析,获得了柞蚕核型多角体病毒基因组的2个极早期基因ie-2和pe-38的序列及pe-38的5′启动子区,其推定的开放阅读框分别编码295和302个氨基酸,并且内部含有一个保守的锌指结构和亮氨酸拉链。
The cells could also grow in serum-free medium SF-900Ⅱ The homologous Antheraea pernyi nuclear polyhedrosis virus was used to infect Ap-4 cell line.
用同源的柞蚕核型多角体病毒感染细胞后，观察了细胞的病理变化以及病毒多角体的形成。
Study on Bombyx mori Nuclear Polyhedrosis Virus (BmNPV) Ubiquitin and Its Binding Peptides;
家蚕核型多角体病毒遍在蛋白及结合肽的研究
Study on Bombyx Mori Nuclear Polyhedrosis Virus (BmNPV) Ubiquitin and Purification of Bombyx Mori Ubiquitin;
家蚕核型多角体病毒遍在蛋白及家蚕遍在蛋白的分离纯化
Resistance to BmNPV of Transformation Cells Expressing Short lef-1 dsRNA
表达短lef-1 dsRNA的转化细胞对家蚕核型多角体病毒的抗性
Cloning and Sequence Analysis of helicase Gene of Philosamia cynthia ricini nucleopolyhedrovirus;
蓖麻蚕核型多角体病毒解旋酶基因的克隆和序列分析
To obtain more molecular information of Philosamia cynthia ricini nucleopolyhedrovirus(PcrNPV),some fragments of PcrNPV DNA were sequenced and analyzed.
基于丰富蓖麻蚕核型多角体病毒(Philosamia cynthia ricininucleopolyhedrovirus,PcrNPV)分子信息的目的,对PcrNPV DNA部分片段进行了测序分析,获得1个DNA结合蛋白基因p6。
【Objective】Studies were performed to investigate the inhibitory effects of different corresponding dsRNAs of gene gp64 on the multiplication of Bombyx mori Nucleopolyhedrovirus (BmNPV).
【目的】研究gp64基因相对应的多个dsRNA对家蚕核型多角体病毒增殖的抑制效果。
Antheraea pernyi nuclear polyhedrosis virus
柞蚕核型多角体病毒
Analysis of Oral Infection and Helicase Gene of the Nucleopolyhedroviruses Isolated from Philosamia Cynthia Ricini and Antheraea Pernyi;
蓖麻蚕和柞蚕核型多角体病毒经口感染及其解旋酶基因的分析
ApNPV Molecular Biology and the Characterstic of Baculoviras Infection;
柞蚕核型多角体病毒分子生物学及杆状病毒感染特性
Research Progress on Antheraea Pernyi Nuclear Polyhedrosis Virus of Tussah and Prevention Methods
柞蚕核型多角体病毒病及其防治方法的研究进展
Construction of Antheraea Pernyi Nuclear Polyhedrosis Virus Double Promoter Transfer Vector Contains Genes of IGF-1 and GFP;
构建携带IGF-1和GFP基因的柞蚕核型多角体病毒双启动子转移载体
Bombyx mori nuclear polyhydrosis virus
家蚕核型多角体病毒
Bombyx mori single nuclear polyhydrosis virus
家蚕单（核壳体）核型多角体病毒
Characterization of the Open Reading Frame 4 of Bombyx Mori Nucleopolyhedrovirus (BmNPV);
家蚕核型多角体病毒ORF4的研究
Characterization of ORF67 and ORF101 from Bombyx Mori Nucleopolyhedrovirus;
家蚕核型多角体病毒（BmNPV）ORF67、ORF101基因分析
Characterization of ORF67 and Three Other Genes from Bombyx Mori Nucleopolyhedrovirus;
家蚕核型多角体病毒ORF67等基因分析
Characterization of Bm41 and Bm51
家蚕核型多角体病毒Bm41和Bm51基因分析
Nucleotid sequence analysis on the p35 gene of Bombyx mori nuclear polyhedrosis virus;
家蚕核型多角体病毒p35基因的核苷酸序列分析
Bombyx Mori L. Nucleopolyhedrovirus (BmNPV) and Proteome Analysis of Silkworm Proteins Related to BmNPV Infection Resistance
家蚕核型多角体病毒与家蚕抗病毒相关的蛋白质组分析
Studies on the Actias selene Multiple-enveloped Nuclear Bolyhedrasis Virus
柳天蚕蛾多粒包埋核型多角体病毒的研究
Joint Pathogenicity of Bacillus Thuringiensis and Bombyx Mori Nuclear Polyhedrosis Virus to Silkworms;
苏云金杆菌与家蚕核型多角体病毒对家蚕联合致病性的研究
Comparative Studies on Two Strains of Nuclear Polyhedrosis Viruses of Philosamia Cynthia ricini (Attacus ricini)
两株蓖麻蚕核型多角体病毒的比较研究
Cloning and Expression of Nucleopolyhedrovirus ORF75 Gene to Bombyx Mori;
家蚕核型多角体病毒ORF75基因的克隆及表达
Location of Fibroblast Growth Factor from Bombyx Mori Nuclear Polyhedrin Virus in Cells and Organs;
家蚕核型多角体病毒FGF 的细胞和组织定位研究