The DNA fragment encoding the truncated SLT-IIeB and FedF of Ee strain were fused to the downstream of glutathione S-transferase (GST) of pGEX-KG expression vector, resulting in the fusion expression plasmid pKSF.
根据猪水肿病大肠杆菌Ee株主要免疫原性片段SLT-IIeB和FedF的基因序列,利用PCR技术克隆目的片段,将SLT-IIeB和FedF基因依序串联于谷胱甘肽-S-转移酶(GST)表达系统的GST下游,在大肠杆菌中成功获得了大小约为63kDa融合蛋白GST-SF,Western blot检测证实表达的融合蛋白具有良好的生物学活性。
Determination of the ee values and content of β-amino alcohol chiral drug intermediates by HPLC
高效液相色谱法测定β-氨基醇类药物中间体的含量及ee值
Determination of the ee values and content of β-amino alcohol chiral drug intermediates by HPLC
高效液相色谱法测定β-氨基醇类药物中间体的含量及ee值
The Research on the Implementation of OTA System Based on Java EE Platform
OTA系统的Java EE实现研究
Fundamental Researches of Separating EPA-EE and DHA-EE by Supercritical Fluid Chromatography;
超临界流体色谱分离EPA-EE和DHA-EE的基础研究
Web Service Security Research Based on Java EE;
基于Java EE的Web服务安全性研究
Auto-Construction On the SQL Statements in Java EE
Java EE中SQL语句的自动构造
Research on Quick Development of Java EE Based on EXT
基于Ext的Java EE快速开发研究
The Design and Implement of Project Management System Based on JAVA EE Platform;
基于JAVA EE平台的项目管理系统的设计与实现
Research of Enterprise Legacy System Integration Scheme Based on Java EE Platform;
基于Java EE平台的企业遗留系统集成方案研究
Developing Spy Tool Based on Symbian and Java EE Platform;
基于Symbian和Java EE平台的手机侦查工具开发
Development and Research of Java EE-based Remote Diagnose System;
基于Java EE平台的远程诊断系统的开发和研究
Research on Data Exchange Technology of Logistics Based on XML and Java EE;
基于XML和Java EE的物流数据交换技术的研究
The Application of Java EE Pattern and Framework in Medium and Small Scale Enterprises;
Java EE模式框架在中小企业Web实施应用中的研究
Research and Application of Java EE Rapid Web Development Framework Based on Reuse;
基于复用的Java EE快速Web开发架构的研究与应用
The Study and Establishment of the Open Campus Network IT Operation Platform Based on Java EE
基于Java EE的开放式校园网IT运维平台的研建
Research on Software Testing in Light-Weight Java EE Framework
轻量级Java EE框架下软件测试的应用研究
Design and implementation of persistence layer's solutions based on Java EE
Java EE数据持久层解决方案的设计与实现
Basic environmental testing procedures for electric and electronic products-Test Ee: Bounce
GB/T2423.39-1990电工电子产品基本环境试验规程试验Ee:弹跳试验方法
Pronounced with the tongue near the palate, as the ee in meet.Used of vowels.
闭元音的舌靠近颚发出声音的，如ee在meet中。用于元音