To establish the technique of RT nested PCR for diagnosis of hemorrhagic fever with renal syndrome(HFRS), and typing of the amplified product was carried out by restriction enzyme digestion.
为建立检测肾综合征出血热 (HFRS)患者血清中汉坦病毒 (HV)基因的RT nestedPCR方法 ,并进一步进行限制性酶切分型 ,设计并合成互补于HVS基因片段的引物 ,对 78例HFRS患者血清进行RT nestedPCR检测 ,并对PCR产物进行酶切分析。
complete digestion
完全消化[多指限制酶切反应]
Restriction Enzyme Mediated Integration, a New Strategy for Cloning New Genes
限制酶介导整合技术:一种克隆新基因的新策略
Study on Limited Enzymatic Hydrolysis of Soy Protein Concentrate and Soy Protein Isolate and Modification in Functional Properties;
大豆浓缩蛋白和分离蛋白限制酶解及功能性变化
circular restriction map
环形限制（酶切）图（谱）
Restriction enzymes and reverse transcriptase are keys to gene engineering.
限制性酶和逆转录酶成为基因工程的重要手段.
Studies on the Active Groups of Pst I Restriction Endonuclease
限制性核酸内切酶PstⅠ活性基团的研究
Methods The restriction endonuclease and T4 DNA ligase were used to construct the vector plasmid.
方法载体的构建采用限制性内切酶酶切、4DNA连接酶连接等方法。
Research of Reaction Mechanism and Kinetics Analysis for the FokI Restriction Endonuclease;
限制性内切酶FokI作用机制研究及动力学分析
Secondly, special enzymes called extinction enzymes are used to cut the DNA at specific places.
第二，利用被叫做限制性内切酶的特殊的酶在特定地方切断DNA。
The distribution of polymorphism at restriction enzyme site-48 A/ G was analyzed with the method of polymerase chain reaction-restriction fragment length polymorphism( PCR-RFLP).
用聚合酶链反应-限制性片段长度多态性方法,分析限制性酶切位点-48A/的多态分布。
Guangzhou Enzyme Products Factory
广州酶制品厂/广州市华琪生物科技有限公司
Partially hydrolysis of de-gossypol cottonseed protein with trypsinase
胰蛋白酶限制性水解脱酚棉籽蛋白的研究
Effects of limited proteolysis on functional properties of soy 11S glycinin
限制性酶解对大豆11S球蛋白功能特性的影响
Development and Clinical Application of SARS Virus cDNA Restriction Endoenzyme Analysis Kit;
SARS病毒cDNA限制性内切酶检测试剂盒的研制和临床应用研究
New phages can develop by acquiring restriction enzymes from plasmids.
通过从质粒获得的限制性酶,新的噬菌体能够生长。
Large DNA molecules are first dissected with restriction enzymes to produce specific fragments.
首先用限制性内切酶将大的DNA分子切断，产生出特殊的片段。
It enables a specific gene to be located on a particular restriction enzyme fragment.
它就能使专一的基因被定位于特定的限制性内切酶切成的片段上。
Construction of Protoplast-Based Transformation System of Fungal Biocontrol Agent Paecilomyces Fumosoroseus with Restriction Enzyme-Mediated Integration (REMI);
限制性内切酶介导的玫烟色拟青霉原生质转化体系构建