Objective To investigate whether the expression of CD59 on the surface of the mutant K562 cells could be reversed by the PIG-A gene mediated by the retrovirus vector.
方法采用PCR方法从质粒pEBPIG-A中扩增PIG-A基因片断,BamH I及Xho I双胁切后连接至逆转录病毒载体pLEGFP-C1相应位点。
The PIG-A gene mutation in PNH patients was assessed with reverse transcription-polymerase chain reaction, het-eroduplex analysis (RT-PCR-HA) and polyacrylamide gel electrophoresis (PAGE).
方法:选取PNH患者8例,正常对照组15例,以逆转录-聚合酶链反应异源双链分析银染法进行PIG-A基因检测,流式细胞术检测外周血细胞表面CD47、CD59的表达。
PIG cleaning examples in water injection pipeline cleaning of Zhongyuan Oil Field
中原油田注水管线PIG清洗实例
After research and practices, the PIG cleaning and a method integrated PIG cleaning withchemical cleaning were succesfully applied to the bittern water pipeline in the salt mineral factory.
通过探索性实践,将PIG清洗以及化学清洗与PIG清洗结合的方法成功应用于某盐矿的卤水输送管线的清洗。
Maize immature embryos were bombarded via a PIG gene gun with plasmid DNAs that contained cryIA(b) gene and bar gene respectively .
利用PIG基因枪 ,将cryIA(b)基因和bar基因 ,采用共转化法导入玉米自交系的幼胚中 ,经PPT筛选 ,获得抗性愈伤组织并再生植株。
This paper introduces an apparatus used for producing atomic oxygen,which consists of a PIG ion source with permanent magnet,two electrodes extraction system, an electron deflector,an einzel lens,an ion decelerating electrode and a sample bracket.
本文介绍一种基于氧负离子PIG源的原子氧产生装置,本装置由永磁PIG离子源、两电极引出系统、电子过滤器、单透镜、减速电极和样品架组成。
Application of PIG cleaning technology in steam pipeline cleaning
PIG清洗技术在蒸汽管道清洗中的应用
Structural Design and Experimental Study of New Cleaning Pig
一种新型管道清洗Pig结构设计及实验研究
cabinet type washer
柜式清洗机.箱式清洗机
hydrodemolition equipment [for cleaning tunnels]
高压清洗机〔清洗隧道〕
Wash often. Keep clean.
常清洗、保持清洁。
Clean out laundry tub after use.
洗完衣服后清洁洗涤槽。
water dispersing power in dry cleaning solvents
干洗清洗剂中水分散度
To dress(a fowl).
宰杀并清洗（家畜）
clean-up period
(机器)清洗周期(两次清洗的间隔时间)
The drive cannot be cleaned or does not support cleaning.
无法清洗驱动器或不支持清洗。
"This cleaning cartridge can perform 1 more drive cleaning.
"该清洗磁带能再清洗一个驱动器。
cleanse with a cleaning agent, such as soap, and water.
用肥皂和水等清洗剂来清洗。
The application of Multienzyme abluent in washing in the endoscopic digesting.
多酶清洗剂在消化内镜清洗中的应用
To free from dirt, defilement, or guilt; purge or clean.
使清洁，清洗使免除灰尘、玷污或罪责；洗净或清除
Comparative Research on Cleaning Effect between Multienzyme Abluent of RUHDF Combined with Des-rust Abluent and Multienzyme Abluent of RUHDF
RUHDF多酶清洗剂加去锈剂与多酶清洗剂清洗效果的对比研究
A vessel, stone basin, or trough used for washing.
洗涤盆用于洗涤的器皿、石盆或用来清洗的水槽
Kitchen cleaning is one of the biggest household cleaning chores.
清洗厨房算是一项家庭洗洗涮涮的重大工程。
Dishwashing detergent is safe to use as car wash.
洗碗用的洗洁精同样安全适用于清洗汽车。