The engineered gene sXYLA encoding xylose isomerase with high activity coupling xylulokinase gene XKS1 fro.
在此基础上将改造后具有良好特性的木糖异构酶基因sXYLA和来自酸酒酵母的木酮糖激酶基因XKS1耦联,构楚得到重组表达质粒pYX-sXYLA- XKS1,在酿酒酵母YPH499中实现组成型共表达。
The xylulokinase gene XKS1 was cloned from Saccharomyces cerevisiae NAN-27 and ligated into plasmids pMA91 and YEp24, producing pMA-xy203 and YEpP-xy204, respectively.
并在此基础上以共转化策略超表达下游关键酶木酮糖激酶基因XKS1。
In this research, we clone Pichia stipitis xylose reductase gene,xylitol dehydrogenase gene and xylulokinase gene,express them in Saccharymyces cerevisiae to dredge xylose metab.
本研究将毕赤树干酵母的木糖还原酶基因、木糖醇脱氢酶基因和木酮糖激酶基因同时引入酿酒酵母并表达,从而达到疏通酿酒酵母木糖代谢途径,提高木糖利用率和乙醇产率的目的。
To investigate the function of tomato dxs gene and confirm it can facilitate the accumulation of the downsteam metabolites,the coding sequence of dxs gene(ledxs)was cloned from tomato by reverse-transcription polymerase chain reaction(RT-PCR),and constructed into the prokaryotic expression vector pTrcLeDXS.
用RT-PCR(反转录-聚合酶链式反应)的方法从番茄中克隆到5-磷酸脱氧木酮糖合成酶(Deoxyoxylulose-5-phosphate synthase,DXS)基因编码区(记为ledxs),并构建了ledxs原核表达载体pTr-cLeDXS。