Objective: To clone the mecA fragment which encodes the transpeptidase domain of penicillin binding protein 2a(PBP2a) of methicillin resistant Staphylococcus aureus(MRSA),to express and purify the transpeptidase domain of PBP2a.
目的:对编码耐甲氧西林金黄色葡萄球菌(MRSA)青霉素结合蛋白2a(PBP2a)转肽酶区的mecA基因片段进行克隆、表达、纯化及鉴定。
Deletion alanine insulin is further changed to thronine tert butyl ester in the B chain s acid end through transpeptidation reaction with the catalysis of lyticus protease.
进一步在水／有机溶剂介质中该酶的转肽反应，使Ｂ链生成苏氨酸叔丁酯羧基末端，水解去酯后产生人胰岛素。