Strain NG1394 was obtamed by selecting KmrCms colonies,which had lost plashed PBF101 and into which transposition of Tns-nifA had occurred.
为了改变细菌固氮的遗传性状,本实验把嵌合质粒PBF101GI进催娩克氏杆菌NG13后,通过Tns的转座(作用),将基因nifA整入受体菌基因组,而获得了在高氨条件下具有稳定固氮能力的菌株NG1394,并对其固氮特性进行分析。
On the basis of the mechanism of transposition, transposable elements were divided into two classes.
转座子是染色体上可移动的DNA序列,根据转座机制可将其分为:通过RNA中间体进行转座的逆转录座子(Retrotransposon)和通过DNA中间体进行转座的转座子(Transposon)。
The gene deletion technique include transposition brack.
其中基因缺失技术还包括转座子插入法和自杀性质粒的构建。
A twin ovary mutant derived from the doubled haploid (DH) progeny of a cross,02428/Gui 630, was presumably related to the transposition of some transposable elements.
以水稻双子房突变体的总DNA为模板 ,用简并引物扩增类copia逆转座子的逆转录酶区域。
According to the character of medium-frequency quenching for revolving seat ring race in military gun turret,the quenching process parameter of decreasing the seat ring reformation and its process method,which can provide the technological guarantee to the production of gun turret seat ring,have been obtained through studying on the quenching process for race and the formation of seat ring.
根据某军品炮塔回转座圈滚道中频淬火的特点,通过对滚道淬火工艺及座圈变形的研究,得出减小座圈变形的淬火工艺参数及工艺方法,为炮塔座圈的生产提供了技术保障。
Detection of Transposon Tn5281 Mediating High-level Gentamicin Resistance in Enterococcus faecalis;
粪肠球菌耐高浓度庆大霉素转座子Tn5281的检测
Insertion mutagenesis in Citrobacter freundii by Mini-Tn5 transposon;
利用Mini-Tn5转座子诱导弗氏枸橼酸杆菌插入突变的研究
Identification of genes for biofilm formation in a Salmonella enteritidis strain by transposon mutagenesis;
转座子随机插入鉴定肠炎沙门氏菌生物膜形成相关基因
A sacB mutant was obtained by transposon IS10 inactivation of a plasmid pXT3sacB carrying the sacB gene.
转座因子在生物体内广泛存在,它在研究基因的重组机理以及生物染色体的进化方面有着重要意义。
Correlation between Mariner transposable element and resistance to Plutella xylostella L.;
Mariner转座子与小菜蛾抗性关系的研究
Objective Use the Drosophila mauritiana mariner transposable element as a vector to construct Mycobacterium smegmatis random mutagenesis library in order to screen transposon mutants that affect the metabolism of fatty acids.
目的 利用果蝇mariner转座子作为载体构建土垢分枝杆菌随机变异基因文库以筛选影响脂肪酸代谢的基因。