Isolation of inulinase production strain and optimization of its fermentation condition;
产菊粉酶菌株的筛选及其产酶条件的优化
Properties and continuous hydrolysis of inulin by immobilized inulinase on cotton cloth;
棉纤维固定化菊粉酶酶学性质及连续降解菊糖的研究
Isolation and purification of inulinase from Aspergilius niger Uγ-2;
黑曲霉Uγ-2胞外菊粉酶的分离纯化
Specific studies content and result were as follows:(1) Inulase can catalyze levan of Jerusalem artichoke hydrolyze as fructose or fructooligosaccharides.
本文主要采用透明圈初筛等方法筛选出一株黑曲霉A18(Aspergilhts niger 18),采用响应面分析方法优化液体发酵产得外切菊粉酶,利用外切菊粉酶作用于菊芋提取液制备菊芋提取液;然后响应面分析法优化黑曲霉发酵生产菊粉酶的发酵工艺条件。
The high production inulase strain was screened from the soil sample where burdock was planted in Qin Village,Bayou Town,Pei County,Xuzhou.
从徐州沛县河口镇秦庄村牛蒡种植基地取得的土壤样本中,筛选出产菊粉酶的菌株,对从土壤中分离到的40株产菊粉酶的各类菌株进行酶活的测定。
A mutant AL 154 Inulase activity reaches 146.
菊芋富含菊粉，菊粉经菊粉酶一步酶解，可生产高果糖浆。
The expression of exoinulinase from Kluyveromyces marxianus in Pichia pastoris;
克鲁维酵母中外切菊粉酶基因在毕赤酵母中的表达研究
Three of them were identified as exoinulinase,and the rest one mainly contained endoinulinase.
采用硫酸铵分级沉淀、透析脱盐、DEAE-Cellulose离子交换色谱等分离纯化技术,从As-pergillus ficuum菌株混合酶系中分离得到4种菊粉酶组分,应用薄层层析法分离各组分水解菊粉的产物,发现其中3种组分主要含外切菊粉酶,一种主要含有内切菊粉酶。
Proved using TLC and HPLC, three bands had exoinulinase activity and two bands had endoinulinase activity.
采用活性聚丙烯酰胺凝胶电泳法对Aspergillusficuum产果聚糖酶体系进行了分离 ,获得8条谱带 ;进一步运用薄层色谱 (TLC)和高效液相色谱 (HPLC)法进行分析 ,发现 8条谱带中有 3条属于外切菊粉酶 ,2条属于内切菊粉酶 ,证明了Aspergillusficuum能同时产内切菊粉酶和外切菊粉酶 。
Purification and Properties of Endoinulinase from Chaetomium sp.;
毛壳霉内切菊粉酶的纯化与性质
Three of them were identified as exoinulinase,and the rest one mainly contained endoinulinase.
采用硫酸铵分级沉淀、透析脱盐、DEAE-Cellulose离子交换色谱等分离纯化技术,从As-pergillus ficuum菌株混合酶系中分离得到4种菊粉酶组分,应用薄层层析法分离各组分水解菊粉的产物,发现其中3种组分主要含外切菊粉酶,一种主要含有内切菊粉酶。
Proved using TLC and HPLC, three bands had exoinulinase activity and two bands had endoinulinase activity.
采用活性聚丙烯酰胺凝胶电泳法对Aspergillusficuum产果聚糖酶体系进行了分离 ,获得8条谱带 ;进一步运用薄层色谱 (TLC)和高效液相色谱 (HPLC)法进行分析 ,发现 8条谱带中有 3条属于外切菊粉酶 ,2条属于内切菊粉酶 ,证明了Aspergillusficuum能同时产内切菊粉酶和外切菊粉酶 。
Study on the INU1 gene recombinant Pichia pastoris GS115 for hydrolysis of sucrose;
菊粉酶基因重组酵母菌水解蔗糖的研究
The Hydrolysis of Inulin with Immobilized Inulinase on Cotton Cloth;
棉织物固定化菊粉酶酶解菊粉的研究
Chemical Modification of Aspergillus ficuum Inulinases
Aspergillus ficuum菊粉酶的化学修饰
Studies on the Screening of Filamentous Fungi and the Production of Inulinase;
产菊粉酶丝状真菌的筛选及其菊粉酶的研究
Characteristics and Enzyme System of Inulinase from Penicillium sp.B01
青霉菌B01产菊粉酶特性的研究及菊粉酶系分析
Hydrolysis of Jerusalem Artichoke by Inulinase Degrading Microorganism;
产菊粉酶微生物水解菊芋粉的应用研究
The studies on enzyme system of Aspergillus niger strains with high inulinase activity
高产菊粉酶的黑曲霉菌株产酶酶系分析与研究
The Study of Solid-state Fermentation for Inulinase Production and Its Protective Agent
菊粉酶的固态发酵及其保护剂的研究
Intrinsic Fluorescence of Aspergillus ficuum Inulinases
Aspergillus ficuum菊粉酶的荧光光谱研究
Chemical modification and fluorescence spectrum of inulinase from Aspergillus ficuum
Aspergillus ficuum菊粉酶的化学修饰和荧光光谱
Studies of Inulinase Production and Characterization by Renicillium Sp.B01 Isolated from Soil;
产菊粉酶菌株的筛选及其酶学特性的研究
Studies on Inulinase Production by Aspergillus Usamii and Enzyme Properties;
宇佐美曲霉产菊粉酶及其酶学特性研究
Enzymatic Characteristics of Inulinase from Aspergillus niger inu-8
一株黑曲霉菌株所产菊粉酶的酶学性质
Study on Processing Technology of FOS in Jerusalem Artichoke by Endo-inulinase from Aspergillus Niger;
黑曲霉内切菊粉酶生产菊芋低聚果糖工艺及应用研究
Optimization and Kinetics of Enzymatic Hydrolysis of Aspergillus ficuum Endoinulinase
Aspergillus ficuum内切菊粉酶的酶解条件优化及酶解动力学研究
Purification and Chemical Modification of the Thermostable Inulinase from Bacillus Smithii
Bacillus smithii产耐热菊粉酶的分离纯化及其化学修饰
Immobilization of Inulinase and Research on Product of Fructooligosaccharides
菊粉酶的固定化及制备低聚果糖的工艺研究
Optimization of Inulinase Production from Aspergillus ficuum by Solid-state Fermentation
无花果曲霉固态发酵产菊粉酶的工艺条件优化
On the Screening and Fermentable Condition for Inulinase by Filamentous Fungi;
产菊粉酶丝状真菌菌株的筛选及产酶发酵条件的研究