Application of Detergent/polymer Aqueous Two Phase Systems for Purification of Membrane Protein;
去污剂/聚合物双水相体系在膜蛋白分离中的应用
Methods: Glioma cells were split with two groups of detergent (1)2% Triton X-114;(2)1% SDS, 1% Deoxycholic acid Na salt, 1% NP-40, 1mmol/L EDTA to extract cell proteins respectively, then SDS-PAGE was adopted to separate the proteins and Western-Blot was adopted to identify the specific protein.
方法 :分别以两组去污剂 (1) 2 % Triton X- 114;(2 ) 1% SDS、1%去氧胆酸钠、1% NP- 40、1mmol/L EDTA裂解 SHG- 44细胞 ,提取细胞蛋白 ,再以 SDS- PAGE法分离蛋白 ,并以 Western- Blot法分析鉴定。
However,it is hard to get sufficient high quality protein for crystallization,together with its instability in detergent solution,which had hampered high-resolution structure determination of GPCRs.
近年来,随着新型膜蛋白表达体系、新型去污剂、膜蛋白纯化及结晶技术的发展,使得G蛋白偶联受体结构解析工作取得了可喜的进展,也为进一步解析更多GPCR精细结构及相关药物研发奠定重要基础。
The thylakiod membranes isolated from Undaria pinnatifida and Ulva pertusa were solubilized by different detergents SDS, Dig and Triton X-100 at different concentrations.
以褐藻裙带菜(Undaria pinnatifida)和绿藻石莼(Ulva pertusa)为实验材料,利用SDS,Dig,Triton X-100三种去污剂,分别与叶绿素按不同比例增溶类囊体膜,采用蔗糖密度梯度超速离心的方法分离色素蛋白复合物,蔗糖密度梯度为60%,50%,40%,30%,20%,15%和10%。
The effects of different concentrations of detergents SDS and Triton-X 100(TX) on thylakoid’s membrane structure and function in spinach were studied in this paper.
研究了不同浓度的去污剂十二烷基磺酸钠(SDS)和聚乙二醇辛基苯乙醚(Triton-X 100,TX)对菠菜类囊体膜的结构与功能的影响。
This detergent can remove stubborn stains.
这种去污剂能去除难洗的污渍。
This famous household cleaner works wonderfully in removing stains.
这种名牌去污剂，去除污渍，效果显著。
Decontamination of radioactively contaminated surfaces Testing of decontamination agents for textiles??
放射性污染表面的去污? 对纺织品去污剂的试验
An abrasive or filler used in a soap or detergent.
增清剂肥皂或去污剂中用的研磨剂或填料
Decontamination of radioactively contaminated surfaces--Testing method of decontamination agents for textiles
GB/T15850-1995放射性污染表面的去污纺织品去污剂的试验方法
This new kind of detergent is of extra strong.
新式去污剂，具有特效强力。
lithium diiodosalicylate
二碘水杨酸锂[可用作去污剂]
Brij 35 detergent
[商]Brij 35去污剂[一种非离子型去污剂，即十二烷基聚乙二醇醚，或称聚氧
Alkaline detergents are effective for emulsification and deflocculation.
碱性去污剂具备有效的乳化和反絮凝作用。
A machine or substance used in cleaning.
清洁器，去污剂用于清洁工作的机器或物质
Have you tried that new stain remover?It works like magic.
你使用过那种新的去污剂吗？效果非常好。
Effects of Detergents on the Structure and Function of the Thylakoid Membrane of Spinach
去污剂对菠菜类囊体膜结构与功能的影响
an ore of boron consisting of hydrated sodium borate; used as a flux or cleansing agent.
一种由含水硼酸钠组成的硼矿石，用作助熔剂或去污剂。
Influence of Surfactants and Antistatic Agents on Detergency of Dry-Cleaning Agents
表面活性剂及抗静电剂对干洗剂去污力的影响
No detergent can shift these stains.
任何去垢剂都不能除掉这些污迹。
Determination of detergency for laundry detergents
GB/T13174-1991衣料用洗涤剂去污力的测定
A liquid preparation in which laundry is presoaked.
去污迹浸洗剂衣服可预浸在其中的液体制剂
Removal of Cadmium and Chromium from Municipal Sludge by Surfactant and Chelators;
表面活性剂与螯合剂对城市污泥中Cd、Cr的去除